Vitamin B8 (biotin) dosage

Vitamin B8, better known as biotin , is a water-soluble vitamin essential for the body. It plays a role in numerous metabolic functions, particularly in the metabolism of carbohydrates, fatty acids, and amino acids. It also plays a key role in maintaining healthy skin, hair, and nails. Therefore, biotin levels are crucial in cosmetic products, dietary supplements, and nutritional formulations designed to support vitality or beauty.

Analytical methods: HPLC, LC-MS/MS and microbiology

YesWeLab offers several validated techniques for the quantitative analysis of biotin, depending on the nature of your samples and the level of precision required:

• HPLC (high-performance liquid chromatography): a method commonly used to quantify biotin in extracts, powders, and simple formulations.
• LC-MS/MS (liquid chromatography coupled with tandem mass spectrometry): offers excellent sensitivity, particularly useful for complex matrices or low concentrations.
• Microbiological method: sometimes required by certain pharmacopoeias or reference materials, particularly for raw materials or products intended for export.

Our partner laboratories carry out these analyses in compliance with ISO 17025 standards, using methods validated according to good analytical practices.

Why measure biotin levels?

Biotin testing serves several strategic objectives:

• Ensure the compliance of fortified products with authorized nutritional claims (e.g., "source of biotin", "contributes to the maintenance of normal skin and hair").
• Validate dermo-cosmetic or hair formulas incorporating vitamin B8 as a beauty active ingredient.
• Controlling the stability of biotin in sensitive matrices (liquid formulations, drinks, plant extracts).
• Meeting regulatory requirements regarding labelling, ingredient declaration or placing on the market.
• Evaluate the quality of raw materials and verify the effectiveness of enrichment processes.

Affected matrices

Biotin analysis is possible on a wide range of products and ingredients, including:

• Dietary supplements: tablets, capsules, powders, syrups
• Functional foods: nutritional bars, fortified drinks, meal replacements
• Plant extracts, enriched yeasts, technical ingredients
• Cosmetics: hair care, serums, revitalizing creams, nail products
• Premixes and animal nutrition products

Each matrix benefits from a suitable extraction and processing protocol, in order to guarantee the reliability of the results.

Sectors of application

Vitamin B8 testing is applicable to many industrial sectors:

• Nutraceuticals: development of supplements targeting beauty, energy or metabolism
• Cosmetics: formulation and quality control of hair care products, anti-hair loss treatments, and conditioners
• Agri-food: control of fortified foods and validation of claims
• Animal nutrition: assessment of vitamin content in feed intended for livestock or pets
• Research and development: optimization of formulations and evaluation of stability over time

Specialized laboratories for reliable results

YesWeLab works exclusively with partner laboratories selected for their technical expertise, accreditations (ISO 17025, COFRAC, etc.), and ability to adapt to your analytical requirements. Depending on your needs—simple testing, regulatory validation, R&D, or quality control—we will guide you toward the most suitable method and laboratory.

Easier management thanks to our digital platform

Since 2020, YesWeLab has simplified access to laboratory analyses through a centralized platform that allows you to:

• Order your analyses online
• Track the processing of your samples in real time
• Download your technical reports
• Benefit from dedicated scientific support

Our team supports you at every stage to guarantee accuracy, compliance and efficiency.

Further analyses recommended

• Vitamin B1 dosage,
• Vitamin B2 dosage,
• Vitamin B6 dosage,
• Vitamin B12 dosage

Get a personalized quote

Add this analysis to your online quote or contact our scientific team. YesWeLab provides rigorous and responsive support in the development, testing, and promotion of your vitamin B8-enriched products.