Vitamin B6 (pyridoxine) dosage

Vitamin B6 measurement, usually in its most common form—pyridoxine —allows us to quantify this water-soluble vitamin, which is essential for proper metabolic function. Vitamin B6 plays a key role in the synthesis of neurotransmitters (dopamine, serotonin, GABA), the production of energy from proteins, and hormonal and immune regulation. Its measurement is essential in functional products, dietary supplements, and formulations intended for specific populations (pregnant women, athletes, the elderly, etc.).

Analytical methods: HPLC and LC-MS for reliable measurement

YesWeLab offers vitamin B6 dosage using two reference techniques, depending on the complexity of the matrix and the level of precision required:

• HPLC (high-performance liquid chromatography) with UV or fluorescence detection after derivatization. This method is reliable for simple to moderately complex matrices such as supplements, extracts, or fortified foods.
• LC-MS (liquid chromatography coupled with mass spectrometry): recommended for trace analyses, complex matrices or strict regulatory requirements.

The analysis can focus on pyridoxine (B6) , but also on its active forms: pyridoxal , pyridoxamine and pyridoxal-5-phosphate (PLP) , particularly in clinical nutrition or in bioavailability contexts.

Why measure vitamin B6?

Vitamin B6 dosage is essential for several reasons:

• Ensure the enrichment and compliance of food products with nutritional claims
• Justify the B6 content in supplements for energy, immunity, mood or hormones
• Assessing the stability of vitamin B6 in complex or oxidation-sensitive formulations
• Control the quality of raw materials (plant extracts, micronutrient mixtures, enriched yeasts)
• Meet regulatory requirements for a product launch or international export

Analyzed matrices

Our partner laboratories support the analysis of vitamin B6 in a wide variety of matrices:

• Food supplements (capsules, tablets, powders, syrups, etc.)
• Plant extracts or enriched yeasts
• Functional drinks, shots, vitamin waters
• Fortified food products (cereals, bars, meal replacements)
• Premix and animal feed
• Cosmetic products containing vitamin B6 (conditioners, hair lotions)

Each matrix is ​​prepared according to suitable protocols in order to efficiently extract vitamin B6 without degradation or interference.

Areas of application

B6 dosage is a key step in the following areas:

• Nutraceutical: formulation of supplements aimed at supporting metabolism, fatigue, the nervous system
• Agri-food: production of enriched or functional foods
• Vegetarian or sports nutrition: control of B vitamin intake
• Animal nutrition: formulation of balanced micronutrient rations
• Cosmetics: integration of vitamin active ingredients into targeted topical treatments

Expert and accredited laboratories

YesWeLab relies on a network of laboratories expert in the analysis of water-soluble vitamins, ISO 17025 accredited and equipped with the most advanced technologies (HPLC-FLD, LC-MS/MS). Each analysis is carried out according to validated and reproducible protocols, allowing to obtain usable results, whether for routine controls, product development or the preparation of regulatory dossiers.

We support you in choosing the method, preparing your samples and interpreting the results, with a high level of scientific expertise.

Simplified analysis management with YesWeLab

Our digital platform allows you to:

• To submit your analysis requests online
• To monitor the processing of your samples in real time
• To receive your results securely
• To communicate directly with our scientific team

Since 2020, YesWeLab has simplified access to vitamin analyses and supported manufacturers in their quality, compliance and innovation challenges.

Further analyses recommended

• Dosage of vitamins B1,
• Dosage of vitamins B2,
• Dosage of vitamin B12,
• Nutritional profile of plant extracts

Request your personalized quote

Add this analysis to your quote or contact our scientific team. YesWeLab supports you every step of the way, with rigor and responsiveness, to ensure the reliability of your enriched products or functional formulations.