Vitamin B6 (pyridoxine) dosage

Vitamin B6 levels, typically measured in its most common form—pyridoxine —are determined by measuring the concentration of this water-soluble vitamin, which is essential for proper metabolic function. Vitamin B6 plays a key role in the synthesis of neurotransmitters (dopamine, serotonin, GABA), energy production from protein, and hormonal and immune regulation. Its measurement is fundamental in functional products, dietary supplements, and formulations designed for specific populations (pregnant women, athletes, the elderly, etc.).

Analytical methods: HPLC and LC-MS for reliable measurement

YesWeLab offers vitamin B6 measurement using two reference techniques, depending on the complexity of the matrix and the level of precision required:

• HPLC (high-performance liquid chromatography) with UV or fluorescence detection after derivatization. This method is reliable for simple to moderately complex matrices such as supplements, extracts, or fortified foods.
• LC-MS (liquid chromatography coupled with mass spectrometry): recommended for trace analyses, complex matrices or strict regulatory requirements.

The analysis can focus on pyridoxine (B6) , but also on its active forms: pyridoxal , pyridoxamine and pyridoxal-5-phosphate (PLP) , particularly in clinical nutrition or in bioavailability contexts.

Why measure vitamin B6 levels?

Vitamin B6 dosage is essential for several reasons:

• Ensuring the enrichment and compliance of food products with nutritional claims
• Justify the B6 content in supplements intended for energy, immunity, mood, or hormones
• Evaluate the stability of vitamin B6 in complex or oxidation-sensitive formulations
• Control the quality of raw materials (plant extracts, micronutrient mixtures, enriched yeasts)
• Meeting regulatory requirements for a product launch or international export

Analyzed matrices

Our partner laboratories handle the analysis of vitamin B6 in a wide variety of matrices:

• Dietary supplements (capsules, tablets, powders, syrups…)
• Plant extracts or enriched yeasts
• Functional drinks, shots, vitamin waters
• Fortified food products (cereals, bars, meal replacements)
• Premix and animal food
• Cosmetic products containing vitamin B6 (conditioning treatments, hair lotions)

Each matrix is ​​prepared according to adapted protocols in order to efficiently extract vitamin B6 without degradation or interference.

Areas of application

Vitamin B6 testing is a key step in the following sectors:

• Nutraceuticals: formulation of supplements designed to support metabolism, fatigue, and the nervous system
• Agri-food industry: production of fortified or functional foods
• Vegetarian or sports nutrition: monitoring of B vitamin intake
• Animal nutrition: formulation of rations balanced in micronutrients
• Cosmetics: integration of vitamin-rich active ingredients into targeted topical treatments

Expert and accredited laboratories

YesWeLab relies on a network of expert laboratories specializing in the analysis of water-soluble vitamins, accredited to ISO 17025 and equipped with the most advanced technologies (HPLC-FLD, LC-MS/MS). Each analysis is performed according to validated and reproducible protocols, ensuring usable results for routine testing, product development, or the preparation of regulatory dossiers.

We support you in choosing the method, preparing your samples and interpreting the results, with a high level of scientific expertise.

Simplified analytics management with YesWeLab

Our digital platform allows you to:

• Submit your analysis requests online
• To track the processing of your samples in real time
• To receive your results securely
• To communicate directly with our scientific team

Since 2020, YesWeLab has been simplifying access to vitamin analyses and supporting manufacturers in their challenges of quality, compliance and innovation.

Further analyses recommended

• Vitamin B1 dosage,
• Vitamin B2 dosage,
• Vitamin B12 dosage,
• Nutritional profile of plant extracts

Request your personalized quote

Add this analysis to your quote or contact our scientific team. YesWeLab supports you at every stage, with rigor and responsiveness, to ensure the reliability of your enriched products or functional formulations.