Troxerutin dosage

Troxerutin analysis allows for the quantification of this semi-synthetic flavonoid derived from rutin, known for its vasoprotective, antioxidant, and anti-inflammatory . Used in products targeting circulatory health and formulations intended for venous comfort, troxerutin is among the active compounds subject to rigorous controls to ensure the compliance of flavonoid-based food supplements and medications .

The analysis is carried out by high-performance liquid chromatography (HPLC-UV) or by liquid chromatography coupled with mass spectrometry (LC-MS), two reference methods for the accurate quantification of flavonoids in complex matrices.

Principle and operation of the method

Since troxerutin is a water-soluble derivative of rutin, its extraction is carried out using polar solvents.

• In HPLC-UV , chromatographic separation allows troxerutin to be isolated from other polyphenolic compounds, with detection taking place at a specific wavelength (approximately 360 nm).
• In LC-MS , the molecule is identified according to its mass/charge ratio (m/z), guaranteeing increased specificity and sensitivity , even at very low concentrations.

These techniques allow for reliable and reproducible quantification, essential for formulation, stability or quality control studies of finished products.

Analytical performance and related analyses

HPLC and LC-MS methods offer:

• high sensitivity (detection at a few ng/mL),
• excellent accuracy for solid or liquid matrices,
• and inter-laboratory reproducibility adapted to regulatory requirements.

Troxerutin assays can be supplemented by other flavonoid analyses for a complete characterization of the formulations:

• the dosage of rutin,
• the quercetin dosage,
• the dosage of total flavonoids,
• or the measurement of total polyphenols

These combined analyses make it possible to evaluate the quality and stability of plant extracts and flavonoid-based formulations.

Applications and areas of use

Troxerutin dosage is applicable in various contexts:

• Nutraceuticals : quality control and standardization of circulatory or antioxidant food supplements.
• Phytotherapy : verification of the troxerutin content in standardized plant extracts derived from natural rutin.
• Pharmaceutical : compliance control of drugs and products intended for vasoprotective purposes according to pharmacopeia specifications.
• R&D and formulation : studies of stability, compatibility and efficacy in complex matrices (capsules, tablets, liquid solutions).

Controlling the dosage ensures the consistency of the final product, its regulatory compliance, and its biological efficacy.

The advantages of the YesWeLab service

YesWeLab relies on a network of partner laboratories accredited to ISO 17025 and COFRAC standards, experts in phytochemical analysis and flavonoid quantification. Each sample is directed to the most suitable laboratory based on the matrix, analytical method, and regulatory requirements.

Thanks to its digital analysis platform, YesWeLab simplifies the management of your services: quote requests, sample tracking, and online results delivery. Since 2020, numerous players in the nutraceutical and pharmaceutical sectors have trusted us for the quality control and standardization of their plant extracts.

For any specific troxerutin dosage request, contact our scientific team to obtain a personalized quote and tailored technical support.