Tiliroside dosage

The dosage of tiliroside makes it possible to quantify this glycosylated flavonoid naturally present in several medicinal plants such as linden (Tilia cordata) , rose (Rosa canina) , malva or even chamomile .
A molecule of nutraceutical and cosmetic interest, tiliroside is recognized for its antioxidant, anti-inflammatory, soothing and anti-aging properties . Its dosage is essential for the standardization of plant extracts , quality control and the formulation of food supplements or natural cosmetics .

The analysis is carried out by HPLC-DAD or LC-MS/MS , two reference techniques for the identification and quantification of flavonoids in complex matrices.

Analytical methods and protocols

The dosage of tiliroside is carried out according to a rigorous chromatographic protocol:

• Sample preparation : methanolic or hydroalcoholic extraction, filtration and concentration.
• HPLC-DAD analysis : separation on C18 column with detection at 340 nm, wavelength specific to flavonoids.
• LC-MS/MS analysis : structural identification of tiliroside by specific fragmentation, offering excellent sensitivity for complex extracts.

The results allow the determination of the tiliroside content (mg/g) and the assessment of purity, stability or comparability between batches .
The methods used are based on the recommendations of the European Pharmacopoeia , AOAC or validated scientific publications.

Industrial and scientific applications

Tiliroside dosage finds various applications in several sectors:

• Nutraceutical : standardization of natural extracts with relaxing, anti-stress or antioxidant effects.
• Cosmetics : formulation of soothing and anti-aging active ingredients in creams, serums or after-sun care.
• Botanical and phytochemical research : identification of species and quality control of extracts.
• Phytotherapy : evaluation of the concentration of active ingredients for products based on linden or rose.

This analysis helps to ensure the consistency and conformity of plant extracts used in high added value products.

Affected matrices

• Plant extracts (linden, rose, chamomile, malva, etc.),
• Natural food supplements,
• Relaxing or antioxidant nutraceutical products,
• Cosmetic active ingredients or anti-aging formulations.

Further analyses recommended

For a complete assessment of the polyphenolic and antioxidant profile of plant extracts, YesWeLab recommends combining this assay with:

• Dosage of quercetol , a flavonoid closely related to tiliroside,
• Dosage of rutin , another glycosylated flavonoid of interest,
• Dosage of kaempferol , structural aglycone of tiliroside,
• Dosage of verbascoside , another antioxidant phenolic compound,

YesWeLab expertise

YesWeLab collaborates with a network of ISO 17025 and COFRAC accredited laboratories , experts in chromatographic analyses and characterization of secondary metabolites .
Our teams support players in nutraceuticals , phytotherapy and natural cosmetics formulation, standardization and regulatory compliance projects .

Thanks to the YesWeLab digital platform , you can:

• Order your analyses online,
• Track the progress of your samples in real time,