Tiliroside dosage

Tiliroside analysis allows for the quantification of this glycosylated flavonoid naturally present in several medicinal plants such as linden (Tilia cordata) , rose (Rosa canina) , mallow , and chamomile .
A molecule of interest in nutraceuticals and cosmetics, tiliroside is recognized for its antioxidant, anti-inflammatory, soothing, and anti-aging properties . Its measurement is essential for the standardization of plant extracts , quality control , and the formulation of dietary supplements and natural cosmetics .

The analysis is performed by HPLC-DAD or LC-MS/MS , two reference techniques for the identification and quantification of flavonoids in complex matrices.

Analytical methods and protocols

The tiliroside assay is performed according to a rigorous chromatographic protocol:

• Sample preparation : methanolic or hydroalcoholic extraction, filtration and concentration.
• HPLC-DAD analysis : separation on C18 column with detection at 340 nm, wavelength specific to flavonoids.
• LC-MS/MS analysis : structural identification of tiliroside by specific fragmentation, offering excellent sensitivity for complex extracts.

The results allow for the determination of the tiliroside content (mg/g) and the evaluation of purity, stability , and batch comparability .
The methods used are based on European Pharmacopoeia , AOAC , or validated scientific publications.

Industrial and scientific applications

Tiliroside assay has various applications in several sectors:

• Nutraceuticals : standardization of natural extracts with relaxing, anti-stress or antioxidant effects.
• Cosmetics : formulation of soothing and anti-aging active ingredients in creams, serums or after-sun care.
• Botanical and phytochemical research : identification of species and quality control of extracts.
• Phytotherapy : evaluation of the concentration of active ingredients for products based on linden or rose.

This analysis helps to ensure the consistency and conformity of plant extracts used in high value-added products.

Matrices involved

• Plant extracts (linden, rose, chamomile, mallow, etc.),
• Natural food supplements,
• Relaxing or antioxidant nutraceutical products,
• Cosmetic actives or anti-aging formulations.

Further analyses recommended

For a complete evaluation of the polyphenol and antioxidant profile of plant extracts, YesWeLab recommends combining this dosage with:

• Quercetin assay , a flavonoid closely related to tiliroside,
• Rutin assay , another glycosylated flavonoid of interest,
• Assay of kaempferol , structural aglycone of tiliroside,
• Verbascoside assay , another antioxidant phenolic compound,

YesWeLab's expertise

YesWeLab collaborates with a network of ISO 17025 and COFRAC accredited laboratories , experts in chromatographic analysis and secondary metabolite characterization .
Our teams support nutraceutical , phytotherapy , and natural cosmetics in their formulation, standardization, and regulatory compliance .

Thanks to the YesWeLab digital platform , you can:

• Order your analyses online,
• Track the progress of your samples in real time,