Stigmastadiene dosage

The dosage of stigmastadiene makes it possible to identify and quantify this marker of thermal degradation of phytosterols , formed during excessive refining or deodorization treatments of vegetable oils. Its presence is therefore a reliable indicator of the quality and degree of transformation of edible oils .

This analysis is essential to verify the regulatory compliance of vegetable oils and ensure their traceability , particularly in production, import or quality control processes.

Analytical methods

The dosage of stigmastadiene is carried out by gas chromatography , using two approaches:

• GC-FID (gas chromatography with flame ionization detector) : reference method for the accurate quantification of stigmastadiene in edible oils.
• GC-MS (gas chromatography coupled with mass spectrometry) : allows structural identification and confirmation of compounds in complex matrices.

The analysis generally follows the standardized method ISO 15788-1 / AOCS Cd 26-96 , ensuring the reliability and reproducibility of the results.

Industrial applications

Stigmastadiene testing is particularly useful for:

• , virgin or extracted vegetable oils
• Detect excessive heat treatment during industrial refining,
• Ensure compliance with European regulations (e.g. EC Regulation No. 61/2011 on olive oils),
• Ensure transparency and traceability in agri-food and nutraceutical production chains.

Analyzed matrices

• Vegetable oils (olive, sunflower, rapeseed, palm, soybean, etc.)
• Vegetable fats and oil blends
• Food products enriched with sterols
• Nutritional supplements based on phytosterols

Further analyses recommended

For complete control of the quality and purity of oils , YesWeLab recommends:

• Peroxide index : evaluation of primary oxidation,
• Acid number : measurement of the hydrolysis of triglycerides,
• Iodine index : determination of the degree of unsaturation of fatty acids,
• Cholesterol and phytosterol : complete characterization of the sterolic profile of oils.

YesWeLab expertise

YesWeLab offers you its network of ISO 17025 and COFRAC accredited partner laboratories for purity control, chemical characterization and regulatory compliance of oils and fats.
Our intuitive digital platform makes it easy to request quotes , track samples and view analytical results in complete transparency.

Need accurate, standards-compliant stigmastadiene testing?
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