Pterostilbene dosage

Pterostilbene is a natural phenolic compound belonging to the stilbene family, structurally close to resveratrol. Known for its antioxidant, neuroprotective and cardioprotective properties, it is present in particular in blueberries (Vaccinium spp.), grapes (Vitis vinifera) and certain medicinal plants .

Pterostilbene is measured using HPLC-DAD (liquid chromatography with UV detection) or LC-MS/MS, enabling precise identification and quantification in plant extracts, fruit powders or nutraceutical supplements. This analysis is essential to ensure the quality, stability and compliance of health or anti-aging products.

Pterostilbene: a methylated analogue of resveratrol

Pterostilbene (trans-3,5-dimethoxy-4′-hydroxystilbene) is a methoxylated derivative of resveratrol , with which it shares the main biological activities while exhibiting better bioavailability.

Due to the methyl substitution of its hydroxyl groups, it is more resistant to oxidation and metabolic conjugation, giving it a longer plasma half-life and more stable antioxidant activity .

In nature, pterostilbene is mainly found in:

• blueberry ,​
• the red grape ,
• sandalwood ,​
• and some tropical plants .

Objectives and interest of pterostilbene dosage

The dosage of pterostilbene is an essential step for:

• quantify the active contents in blueberry, grape or medicinal plant extracts,
• standardize nutraceutical formulations,
• check the stability of the compound in mixtures or capsules,
• validate functional claims relating to oxidative stress, cardiovascular protection or cognition,
• ensure the quality conformity of finished products before marketing.

Affected matrices include:

• plant extracts ( blueberry, grape, red wine),
• food supplements for health purposes,
• functional formulations ( capsules, tablets, powders),
• antioxidant or nutraceutical drinks

Analytical methods used

Pterostilbene is analyzed by HPLC-DAD or LC-MS/MS , depending on the complexity of the matrix and the expected levels.

• HPLC-DAD : This chromatographic method allows the separation and quantification of pterostilbene thanks to its characteristic absorption in the UV (approximately 306 nm). It is used for plant extracts and standardized products.
• LC-MS/MS : coupling liquid chromatography with mass spectrometry, this technique allows ultra-sensitive and specific quantification , suitable for complex or low-concentration matrices.

The analysis can be completed by the dosage of resveratrol , quercetol (quercetin) , or even kaempferol , in order to obtain a complete polyphenolic profile .

Industrial and regulatory applications

Pterostilbene dosage applies to several areas:

• Nutraceuticals and food supplements : quality control of standardized extracts and validation of the content of active ingredients claimed in antioxidant, anti-aging or cardiovascular products.
• Plant and functional ingredient industry : characterization of natural extracts rich in stilbenes for nutraceutical ingredient manufacturers.
• Scientific research and R&D : studies of bioavailability, stability, metabolism or synergy with other polyphenols such as resveratrol.
• Natural cosmetics : formulation of anti-aging and antioxidant active ingredients in topical care, linked to the effects of resveratrol on the skin.

Analytical results are essential to support regulatory dossiers , nutritional claims or clinical studies investigating the efficacy of stilbene-enriched products.

Carrying out analyses at YesWeLab

YesWeLab collaborates with a network of partner laboratories specialized in polyphenolic and phytocompound analyses , equipped with cutting-edge equipment (HPLC-DAD, LC-MS/MS, UHPLC).

The tests are carried out using validated methods, in accordance with the requirements of the ISO 17025 , and guarantee complete traceability of the results.

Each request is studied according to the nature of the product, the matrix, the expected thresholds and the applicable regulatory requirements.

Since 2020, YesWeLab has been supporting players in the nutraceutical and natural ingredients sector in the standardization of their plant extracts and the promotion of their antioxidant active ingredients .

The YesWeLab digital platform simplifies the management of your analytical needs: requesting quotes, tracking samples and receiving analysis reports online.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors: Nutraceuticals, plants, natural cosmetics
• Measured parameters: Pterostilbene (methoxylated stilbene)
• Normative references: ISO 17025, validated internal methods

For any specific request, contact our scientific team for a personalized quote and tailor-made technical support.