Pterostilbene assay

Pterostilbene is a natural phenolic compound belonging to the stilbene family, structurally similar to resveratrol. Known for its antioxidant, neuroprotective, and cardioprotective properties, it is found in blueberries (Vaccinium spp.), grapes (Vitis vinifera), and certain medicinal plants .

Pterostilbene levels are measured using HPLC-DAD (high-performance liquid chromatography with UV detection) or LC-MS/MS, allowing for precise identification and quantification in plant extracts, fruit powders, or nutraceutical supplements. This analysis is essential to guarantee the quality, stability, and compliance of health and anti-aging products.

Pterostilbene: a methylated analogue of resveratrol

Pterostilbene (trans-3,5-dimethoxy-4′-hydroxystilbene) is a methoxylated derivative of resveratrol , with which it shares the main biological activities while exhibiting better bioavailability.

Thanks to the methyl substitution of its hydroxyl groups, it is more resistant to oxidation and metabolic conjugation, which gives it a longer plasma half-life and more stable antioxidant activity .

In nature, pterostilbene is mainly found in:

• blueberry ,​
• red grapes ,
• sandalwood ,​
• and certain tropical plants .

Objectives and benefits of pterostilbene assay

Pterostilbene measurement is an essential step for:

• quantify the active compounds in extracts of blueberry, grape or medicinal plants,
• standardize nutraceutical formulations,
• to verify the stability of the compound in mixtures or capsules,
• validate functional claims related to oxidative stress, cardiovascular protection, or cognition,
• to guarantee the quality conformity of finished products before marketing.

The matrices in question include:

• plant extracts ( blueberry, grape, red wine),
• dietary supplements for health purposes,
• functional formulations ( capsules, tablets, powders),
• antioxidant or nutraceutical drinks

Analytical methods used

Pterostilbene is analyzed by HPLC-DAD or LC-MS/MS , depending on the complexity of the matrix and the expected levels.

• HPLC-DAD : This chromatographic method allows the separation and quantification of pterostilbene thanks to its characteristic absorption in the UV range (approximately 306 nm). It is used for plant extracts and standardized products.
• LC-MS/MS : coupling liquid chromatography with mass spectrometry, this technique allows ultra-sensitive and specific quantification , suitable for complex or low-concentration matrices.

The analysis can be supplemented by measuring resveratrol , quercetin , or kaempferol , in order to obtain a complete polyphenolic profile .

Industrial and regulatory applications

Pterostilbene assay is applicable to several fields:

• Nutraceuticals and food supplements : quality control of standardized extracts and validation of the content of active ingredients claimed in antioxidant, anti-aging or cardiovascular products.
• Plant and functional ingredients industry : characterization of natural extracts rich in stilbenes for nutraceutical ingredient manufacturers.
• Scientific research and R&D : studies of bioavailability, stability, metabolism or synergy with other polyphenols such as resveratrol.
• Natural cosmetics : formulation of anti-aging and antioxidant active ingredients in topical treatments, linked to the effects of resveratrol on the skin.

Analytical results are essential to support regulatory dossiers , nutritional claims , or clinical studies on the efficacy of stilbene-enriched products.

Analysis performed at YesWeLab

YesWeLab collaborates with a network of partner laboratories specializing in polyphenolic and phytocompound analyses , equipped with state-of-the-art equipment (HPLC-DAD, LC-MS/MS, UHPLC).

The tests are carried out according to validated methods, in accordance with the requirements of ISO 17025 , and guarantee complete traceability of the results.

Each application is studied according to the nature of the product, the matrix, the expected thresholds and the applicable regulatory requirements.

Since 2020, YesWeLab has been supporting players in the nutraceutical and natural ingredients sector in the standardization of their plant extracts and the enhancement of their antioxidant active ingredients .

The YesWeLab digital platform simplifies the management of your analytical needs: requesting quotes, tracking samples and receiving analysis reports online.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physico-chemical, nutritional
• Sectors: Nutraceuticals, plants, natural cosmetics
• Measured parameters: Pterostilbene (methoxylated stilbene)
• Normative references: ISO 17025, validated internal methods

For any specific request, contact our scientific team for a personalized quote and tailored technical support.