Resveratrol dosage

Resveratrol belonging is a natural polyphenol to the stilbene, known for its antioxidant, anti-inflammatory, and cardioprotective properties. Found in grapes, red wine, Japanese knotweed (Polygonum cuspidatum) , and blueberries, it is a key biomarker in evaluating the quality of plant extracts and nutraceutical products.

Resveratrol is measured using HPLC-DAD or LC-MS/MS, allowing for precise identification and quantification of the compound, whether in its trans-resveratrol (active) or cis-resveratrol (secondary isomer) form. This analysis is essential to guarantee the purity, stability , and standardization of formulations intended for health, antioxidant, or anti-aging purposes.

Resveratrol: a major bioactive polyphenol

Resveratrol (3,5,4′-trihydroxystilbene) is a phenolic compound produced by certain plants in response to environmental stress (infection, UV radiation, drought). It exists in two isomeric forms:

• trans-resveratrol, biologically active and the most sought after;
• cis-resveratrol, less stable and often derived from the transformation of the former.

Its presence is particularly notable in:

• the skin of the grapes and red wine,
• , Japanese knotweedrich in natural resveratrol used for standardized extracts,
• peanuts blueberries, blackberries, . and other colorful fruits

Resveratrol is often studied for its effects on cellular longevity, cardiovascular health and protection against oxidative stress, hence its growing importance in functional nutrition and natural cosmetics.

Objectives and purpose of resveratrol assay

The measurement of resveratrol by chromatography allows us to:

• quantify the total resveratrol content in plant extracts, wines or food supplements,
• evaluate the trans/cis ratio to ensure product stability and efficacy,
• to standardize batches of natural ingredients in nutraceutical supply chains,
• verify the regulatory compliance of enriched formulations,
• support antioxidant and nutritional claims on product labels.

The most commonly analyzed matrices include:

• ( plant extracts particularly Japanese knotweed),
• in dietary supplements capsule, powder or liquid form,
• wines and beverages,
• rich Natural cosmetic products in polyphenols.

Analytical methods used

Resveratrol is analyzed by HPLC-DAD or LC-MS/MS depending on the nature and complexity of the matrix:

• HPLC-DAD (UV-Visible Detection Liquid Chromatography) : the reference method for quantifying resveratrol in plant extracts and finished products. Detection occurs around 306 nm, the characteristic wavelength of stilbene.
• LC-MS/MS (liquid chromatography coupled with mass spectrometry) : a more sensitive technique allowing specific identification of isomers and ensuring reliable quantification even at low concentrations.

This analysis can be supplemented by additional tests such as:

• Pterostilbene assay,
• Total polyphenol content,
• Total flavonoid content,
• Anthocyanin assay,
  to establish a complete antioxidant profile of the sample.

Industrial and regulatory applications

Resveratrol assay is used in many sectors:

• Nutraceuticals : control of resveratrol content in food supplements and plant extracts for health or anti-aging purposes.
• Agri-food and oenology : monitoring of polyphenol levels in wine, grapes or juices, an indicator of quality and antioxidant activity.
• Natural cosmetics : formulation of anti-aging and antioxidant active ingredients in skincare.
• Scientific research : studies of bioavailability, antioxidant efficacy and synergistic interactions with other phenolic compounds (quercetin, catechins, pterostilbene).

From a regulatory standpoint, extracts standardized in resveratrol must meet purity and traceability requirements, particularly within the framework of European regulations on food ingredients and supplements.

Analysis performed at YesWeLab

YesWeLab collaborates with a network of expert laboratories in chromatography of plant polyphenols and metabolitesinstruments HPLC-DAD, UHPLC and LC-MS/MS requirements ISO 17025.

The tests are performed using validated methods, ensuring accuracy, reproducibility , and complete traceability of the results. Analysis reports include:

• the total resveratrol content (mg/g or mg/L),
• the trans/cis distinction,
• and compliance recommendations for industrial applications.

players nutraceutical,agri-food and natural cosmetics in the analytical control of their polyphenolic extracts and in the valorization of their active ingredients.

Thanks to its digital platform, YesWeLab centralizes your analytical needs, from quote request to results receipt, guaranteeing responsive and personalized scientific support.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physico-chemical, nutritional
• Sectors: Nutraceuticals, food processing, plants, natural cosmetics
• Measured parameters: Resveratrol (trans and cis)
• Normative references: ISO 17025, validated internal methods

For any polyphenol analysis or antioxidant profile development needs, contact our scientific team for a personalized quote.