Plumbagin dosage

Plumbagin (or 5-hydroxy-2-methyl-1,4-naphthoquinone) is a naphthoquinone naturally occurring in medicinal plants of the genera Plumbago or Diospyros it possesses biological activities (antibacterial, antitumor), it is potentially toxic at high concentrations.

Its dosage is essential for:

• Securing the use of plant extracts containing plumbagin
• Respect the regulatory limits in cosmetics or phytotherapy
• Compare the concentration according to species, origins or extraction processes
• Check for contamination in food supplements
• Documenting natural formulations (creams, oils, tinctures)

Methodology

The analysis is performed by high-performance liquid chromatography (HPLC) or LC-MS for precise detection, even at trace levels. Depending on the type of matrix, several methods are possible:

• HPLC-DAD : Rapid analysis with UV detection at 254 nm
• HPLC-MS/MS : for complex or low-content matrices
• Extraction using organic solvents, with possible purification on SPE (solid phase)

The method is validated according to ICH criteria (linearity, repeatability, specificity, LOD/LOQ).

Analyzed matrices

• Hydroalcoholic or glycolic extracts of Plumbago zeylanica , Diospyros lotus , etc.
• Mother tinctures or standardized extracts for phytotherapeutic use
• Creams, essential oils, plant-based cosmetic serums
• Dietary supplements in liquid or solid form
• Raw materials derived from potentially toxic medicinal plants

Industrial applications

• Phytotherapy : Controlling Active and Toxic Components
• Natural cosmetics : securing formulations based on Plumbago
• Quality control : batch evaluation or labeling verification
• Pharmacotoxicological research : in vitro studies and regulatory dossiers

Why YesWeLab?

HPLC and LC-MS laboratories for you , accredited according to ISO 17025 , specializing in the analysis of complex and potentially regulated plant compounds. Our teams support you in defining the protocol, choosing the methods, and compiling compliance documentation (cosmetic dossiers, safety data sheets, etc.).

See also:

• Chelidonin dosage
• Aloin dosage