Plumbagin dosage

Plumbagin ( or 5-hydroxy-2-methyl-1,4-naphthoquinone) is a naphthoquinone naturally present in medicinal plants of the genus Plumbago or Diospyros . Although it has biological activities (antibacterial, antitumor), it is potentially toxic at high concentrations.

Its dosage is essential for:

• Safer use of plant extracts containing plumbagin
• Respect regulatory limits in cosmetics or phytotherapy
• Compare concentration according to species, origins or extraction processes
• Check for contamination in food supplements
• Document natural formulations (creams, oils, tinctures)

Methodology

The analysis is carried out by high-performance liquid chromatography (HPLC) or LC-MS for accurate detection, even at trace levels. Depending on the type of matrix, several methods are possible:

• HPLC-DAD : rapid analysis with UV detection at 254 nm
• HPLC-MS/MS : for complex or low-content matrices
• Extraction by organic solvents, possible purification on SPE (solid phase)

The method is validated according to ICH criteria (linearity, repeatability, specificity, LOD/LOQ).

Analyzed matrices

• Hydroalcoholic or glycolic extracts of Plumbago zeylanica , Diospyros lotus , etc.
• Mother tinctures or standardized extracts for phytotherapeutic use
• Creams, essential oils, herbal cosmetic serums
• Food supplements in liquid or solid form
• Raw materials from potentially toxic medicinal plants

Industrial applications

• Phytotherapy : control of active and toxic components
• Natural cosmetics : securing formulations based on Plumbago
• Quality control : batch evaluation or labeling verification
• Pharmacotoxicological research : in vitro studies and regulatory dossiers

Why YesWeLab?

YesWeLab selects for you expert laboratories in HPLC and LC-MS , accredited according to the ISO 17025 , specialized in the analysis of complex and potentially regulated plant compounds. Our teams support you in defining the protocol, choosing methods, and creating compliance files (cosmetic files, safety data sheets, etc.).

See also:

• Chelidonine dosage
• Aloin Dosage