Melatonin dosage

Melatonin is a hormone naturally produced by the pineal gland, regulating sleep-wake cycles. In herbal medicine and nutraceuticals, it is used to promote sleep onset, improve sleep quality, or regulate the circadian rhythm in cases of jet lag. It is also found in certain vegetables (e.g., cherries, rice, corn) and fortified products.

Melatonin measurement is essential for:

• Check the actual content in food supplements
• Validate regulatory compliance, particularly regarding labeling and claims
• Ensuring the reproducibility of formulations
• Supporting the development of relaxing products, functional drinks, or phytotherapeutic solutions

Analytical methods: HPLC-DAD or LC-MS/MS

Melatonin analysis is performed by high-performance liquid chromatography (HPLC) with UV detection (DAD) or by chromatography coupled with mass spectrometry (LC-MS/MS) for increased sensitivity. These methods allow for the precise detection and quantification of the molecule, even at very low concentrations (< 0.1 mg/g).

Our protocols are adapted according to:

• The type of formulation (capsules, tablets, plant extracts, liquids)
• The level of precision required by the specifications
• Product conformity standards in force

Analyzed matrices

Melatonin dosage is available for various substrates:

• Dietary supplements (capsules, tablets, lozenges)
• Fortified functional beverages
• Natural plant extracts
• Products intended for animal nutrition (relaxing or regulating formulas)
• Phytotherapy mixtures in liquid or solid form

Applications and sectors concerned

This analysis is intended for nutraceutical manufacturers, formulators of natural relaxation products, animal nutrition professionals, and distributors of enriched phytocomplexes. It is recommended in the context of:

• From the formulation of new products
• Standardization of plant batches rich in melatonin
• Monitoring the quality and compliance of supplements on the market

Related analyses to discover

For a complete study of your relaxing or plant-based formulas, also explore:

• Free theanine dosage (amino acid) – a relaxing amino acid naturally present in green tea
• Magnoflorine dosage – a plant alkaloid with sedative effects
• Trigonelline dosage – an alkaloid with neuroprotective properties
• Tryptamine dosage – a precursor of serotonin, with neuromodulatory effects

Why choose YesWeLab for melatonin testing?

Since 2020, YesWeLab has been supporting manufacturers in the nutrition, plant-based, and dietary supplement industries in managing their analyses. Thanks to our centralized digital platform and a network of ISO 17025/COFRAC certified partner laboratories, we offer:

• Robust analytical protocols, tailored to your requirements
• A rigorous selection of the laboratory according to your target matrices and thresholds
• A scientific team ready to listen to you and personalize the services

Get a quote or contact our team

Add this service to your quote now or contact our team for a customized study of your melatonin-containing formulations.