Madecassoside dosage

Madecassoside asiatica , a medicinal plant renowned for its healing, anti-inflammatory, and soothing properties. This compound is one of the main active markers of Centella, along with asiaticoside and asiatic acid. Together, these molecules contribute to skin regeneration, stimulate collagen synthesis, and reduce tissue inflammation. Madecassoside levels are now an essential quality indicator for manufacturers of plant extracts, natural cosmetics, and dietary supplements based on Centella asiatica.

Objective and purpose of madecassoside assay

Quantitative analysis of madecassoside allows for the standardization of Centella asiatica plant extracts to guarantee a consistent active ingredient content, control the quality and authenticity of botanical raw materials, verify the stability of cosmetic and nutraceutical formulations over time, and demonstrate the biological efficacy of products containing Centella extracts, particularly in restorative, anti-aging, and anti-stretch mark skincare.
In the nutraceutical field, Centella and madecassoside are also being studied for their neuroprotective and antioxidant effects, especially in the context of cellular aging and oxidative stress.

Analytical methods used

At YesWeLab, madecassoside analysis relies on high-precision chromatographic methods.
The first is HPLC-DAD (high-performance liquid chromatography with diode array detection), the reference method for quantifying saponins in plant extracts and cosmetic formulations.
The second is LC-MS or LC-MS/MS (liquid chromatography coupled with mass spectrometry), a highly sensitive technique that allows for the identification and quantification of madecassoside even in complex matrices such as creams, gels, or tablets.
Extraction is performed using aqueous or hydroalcoholic solutions, depending on the nature of the product (dry extracts, glycolic extracts, cosmetic formulations). These methods comply with the European Pharmacopoeia and ISO 17025 standards, guaranteeing traceability and reliable results.

Industrial applications

Madecassoside assay has applications in several sectors.

Cosmetic

It allows quality control of creams, serums, oils and repairing gels, verification of the content of active ingredients in anti-aging and soothing formulations, as well as evaluation of the stability of extracts in healing and anti-stretch mark treatments.

Nutraceuticals and phytotherapy

The analysis is used for the standardization of dry extracts of Centella asiatica intended for food supplements, the verification of the conformity of plant powders and hydroalcoholic extracts, and the study of correlations between saponin content and biological activity (antioxidant, neuroprotective, anti-inflammatory).

Research and development

Madecassoside assay is also implemented for determining the chemical profile of Centella extracts, comparative studies between species or geographical origins and the validation of new eco-responsible extraction methods.

Affected matrices

Dry or glycolic extracts of Centella asiatica;
standardized plant powders
; herbal food supplements;
creams, gels, oils and skin care products;
cosmetic textile formulations and soothing products

Recommended Related Analyses

For a complete characterization of the saponins and bioactive compounds of Centella asiatica, YesWeLab recommends combining madecassoside analysis with other complementary analyses:
Asiaticoside analysis : another major triterpene saponin in Centella asiatica.
Ginsenoside Rg1 analysis : a bioactive saponin from ginseng, for synergistic formulations.
Verbascoside (acteoside) analysis : an antioxidant phenol often associated with restorative plant extracts.
Tiliroside analysis : a soothing flavonoid used in natural anti-inflammatory products.

Why entrust your analysis to YesWeLab?

Since 2020, YesWeLab has been supporting the cosmetic, plant-based, and nutraceutical industries in mastering their phytochemical profiles. Thanks to its network of ISO 17025/COFRAC accredited partner laboratories, YesWeLab guarantees analyses compliant with international standards, complete traceability from sample to results delivery, a selection of methods adapted to each matrix, and customized scientific support for the validation and formulation of your products.

Need to certify the quality of your Centella asiatica extracts? Contact YesWeLab for a quick quote and certified madecassoside analysis in your cosmetic or nutraceutical products.