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Kaempferol dosage

Kaempferol dosage

Technique(s)
Categories: ,
Sector of activity
Expertise

Physicochemical, Nutritional

CAS No.

520-18-3

Description

Kaempferol testing measures the concentration of this natural flavonoid in plant extracts, plant powders, or dietary supplements. Present in numerous plant species, including tea, broccoli, onions, propolis, and ginkgo, kaempferol is known for its potent antioxidant, anti-inflammatory, and oxidative stress-protective properties.

nutraceutical and phytotherapy manufacturers wishing to guarantee the quality and standardization of their flavonoid-rich plant-based products.

Kaempferol: a quality marker for plant extracts

Kaempferol (CAS No. 520-18-3) belongs to the large family of flavonols, along with quercetin and myricetin. These molecules play a major role in plant defense against external aggressions and, in humans, constitute bioactive compounds of interest for health.

In nutraceutical products, kaempferol is often used as an analytical marker to control the quality of extracts of ginkgo, chamomile, aloe vera, or sophora. Its concentration is correlated with the antioxidant activity and functional value of plant-based formulations.

Assessing its content therefore allows us to:

  • standardize batches of plant extracts,
  • verify compliance with pharmacopoeia monographs,
  • to ensure the reproducibility of biological effects,
  • validate claims related to health, cell protection or vitality.

Analytical methods used

The assay of kaempferol is carried out using high-precision chromatographic techniques, allowing the separation and quantification of flavonoids in complex matrices:

  • HPLC-DAD (high-performance liquid chromatography with diode array detection) : the reference method for the quantification of total or individual flavonoids. It allows the quantification of kaempferol from its specific absorption spectrum in the UV-visible range.
  • LC-MS or LC-MS/MS (liquid chromatography coupled with mass spectrometry) : this approach offers superior sensitivity and allows the identification of kaempferol and its glycosylated derivatives in complex plant extracts, while ensuring excellent selectivity.

Depending on the nature of the matrix, a purification step (liquid-liquid extraction or SPE) may be carried out before analysis to eliminate interferences and improve the reliability of the assay.

YesWeLab also offers complementary analyses to characterize the entire polyphenolic profile of an extract, such as the measurement of total flavonoids , the measurement of total polyphenols , or the measurement of quercetin .

Sectoral benefits and applications

The kaempferol dosage is intended for various industrial players:

  • Nutraceuticals and phytotherapy : standardization of extracts of ginkgo, chamomile, green tea, propolis or red fruits in antioxidant and anti-inflammatory formulations.
  • Food industry : evaluation of flavonoid content in plant ingredients used for functional or fortified products.
  • Research and development : study of the stability of kaempferol during extraction, drying or formulation processes.
  • Natural cosmetics : control of flavonol content in plant extracts used for their protective and soothing properties on the skin.

This analysis can be combined with complementary services such as rutin measurement , myricetin measurement , or resveratrol measurement , allowing a complete evaluation of the antioxidant profile of natural extracts.

Carrying out analyses at YesWeLab

YesWeLab entrusts the measurement of kaempferol to partner laboratories specializing in chromatographic and phytochemical analyses. These laboratories are equipped with state-of-the-art HPLC and LC-MS/MS equipment, guaranteeing the accuracy, sensitivity, and reproducibility of the measurements.

Most of our partners are certified or accredited according to the ISO 17025 standard and the COFRAC reference framework, ensuring compliance and traceability of results.

The YesWeLab scientific team assists each client in choosing the analytical method best suited to their needs: identification of free or glycosylated kaempferol, determination of the complete polyphenolic profile or quality control of standardized extracts.

Thanks to the YesWeLab digital platform, managing analyses is simple and centralized: online quote requests, sample tracking, and rapid receipt of analytical reports. Since 2020, numerous players in the nutraceutical, phytotherapy, and natural ingredients sectors have trusted YesWeLab for the chemical characterization and validation of their plant extracts.

Technical data

  • CAS No.: 520-18-3
  • Methods: HPLC-DAD, LC-MS/MS
  • Expertise: Physicochemical, nutritional
  • Business sectors: Nutraceuticals, plants
  • Measured parameters: Kaempferol and glycosylated derivatives

For any specific request, contact our team to obtain a personalized quote and analytical advice tailored to your plant extracts or formulations.

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