Genistein assay

Genistein is an aglycone isoflavone naturally found in soybeans, red clover, and other legumes. Recognized for its antioxidant, anti-inflammatory, and phytoestrogenic properties, it is frequently used as an active ingredient in nutraceutical products targeting hormonal, cardiovascular, or bone health.

Unlike genistin, its glycosylated form, genistein is directly bioavailable and biologically active. Its precise dosage allows for the standardization of plant extracts, guarantees the active ingredient content in formulations, and validates nutritional and health claims.

Objectives of genistein assay

Measuring the concentration of this molecule is recommended in several industrial and scientific contexts:

• Quality control of soy-based food supplements
• Quantification in standardized plant extracts
• Monitoring bioavailability during clinical studies
• Verification of the actual isoflavone content in enriched formulations
• Selection and evaluation of plant-based raw materials

Available analytical methods

YesWeLab offers two reference methods for genistein analysis:

• HPLC-DAD: Liquid chromatography with UV detection at 260–280 nm, ideal for simple matrices and aglycone assay.
• LC-MS/MS: tandem mass spectrometry, more sensitive and selective, suitable for complex matrices or low concentrations.

Samples are generally subjected to liquid-liquid or hydroalcoholic extraction, with or without prior enzymatic hydrolysis, depending on whether the aim is to quantify the free or total form (free + glycosylated).

Analyzable matrices

The assay of genistein is applicable to many plant or processed matrices:

• Extracts of soy or red clover
• Nutraceutical powders and capsules
• Juices and fortified drinks
• Fermented legume-based products (e.g., tempeh, miso)
• Supplements for women's health or menopause

Industrial applications

• Nutraceuticals: formulation of supplements for anti-aging, joints, bone health, menopause
• Herbal medicine: selection of extracts standardized in isoflavones
• Research: pharmacokinetic study, bioavailability or biological activity
• Cosmetics: integration into anti-aging or regenerating formulations

Why choose YesWeLab?

YesWeLab connects you to a network of specialized partner laboratories, ISO 17025 certified and/or COFRAC accredited, equipped with state-of-the-art analytical equipment. Whether you need a one-off test or comprehensive support for your product development project, our scientific team adapts to your regulatory, technical, or R&D requirements.

Ordering and tracking your analyses is simplified thanks to our digital platform dedicated to professionals in the sector.

Recommended internal links to enrich your analytical approach

• Genistin assay : to quantify the glycosylated form naturally present in soybeans
• Total isoflavone assay : useful for an overview (genistein, daidzein, glycitein)
• Resveratrol dosage: another polyphenol with similar properties
• Analysis of total polyphenols : complementary for evaluating overall antioxidant activity

Do you want to get a quote, ask a technical question, or integrate genistein assay into your quality or development program?
Contact our scientific team now via the YesWeLab platform.