Genistein dosage

Genistein is an aglycone isoflavone naturally found in soybeans, red clover, and other legumes. Known for its antioxidant, anti-inflammatory, and phytoestrogenic properties, it is frequently used as an active molecule in nutraceutical products targeting hormonal, cardiovascular, and bone health.

Unlike genistin, its glycosylated form, genistein is directly bioavailable and biologically active. Its precise dosage makes it possible to standardize plant extracts, guarantee the active ingredient content in formulations, or validate nutritional or health claims.

Objectives of genistein assay

The dosage of this molecule is recommended in several industrial or scientific contexts:

• Quality control of soy-based food supplements
• Quantification in standardized plant extracts
• Monitoring bioavailability in clinical studies
• Verification of actual isoflavone content in enriched formulations
• Selection and evaluation of plant raw materials

Available analytical methods

YesWeLab offers two reference methods for genistein analysis:

• HPLC-DAD: Liquid chromatography with UV detection at 260–280 nm, ideal for simple matrices and aglycone determination.
• LC-MS/MS: tandem mass spectrometry, more sensitive and selective, suitable for complex matrices or low concentrations.

Samples are generally subjected to liquid-liquid or hydroalcoholic extraction, with or without prior enzymatic hydrolysis, depending on whether the aim is to measure the free or total form (free + glycosylated).

Analyzable matrices

Genistein dosage applies to many plant or processed matrices:

• Soy or red clover extracts
• Nutraceutical powders and capsules
• Juices and fortified drinks
• Fermented legume products (e.g. tempeh, miso)
• Supplements for women's health or menopause

Industrial applications

• Nutraceuticals: formulation of anti-aging supplements, joints, bone health, menopause
• Phytotherapy: selection of extracts standardized in isoflavones
• Research: pharmacokinetic study, bioavailability or biological activity
• Cosmetics: integration into anti-aging or regenerating formulations

Why choose YesWeLab?

YesWeLab connects you to a network of specialized partner laboratories, ISO 17025 certified and/or COFRAC accredited, equipped with state-of-the-art analytical equipment. Whether you need a one-off assay or comprehensive support for your product development project, our scientific team adapts to your regulatory, technical, or R&D requirements.

Ordering and tracking your analyses is simplified thanks to our digital platform dedicated to professionals in the sector.

Recommended internal links to enrich your analytical approach

• Genistin assay : to quantify the glycosylated form naturally present in soybeans
• Total isoflavones assay : useful for an overview (genistein, daidzein, glycitein)
• Resveratrol dosage: another polyphenol with similar properties
• Total polyphenol analysis : complementary to assess overall antioxidant activity

Would you like to get a quote, ask a technical question, or integrate genistein testing into your quality or development program?
Contact our scientific team now via the YesWeLab platform.