Rhapontin (stilbene) dosage

The rhapontin assay is a reference analysis for the authentication of rhubarb extracts ( Rheum spp. ) used in natural food supplements. A glycosylated stilbene specific to certain rhubarb species, rhapontin is recognized as a phytological marker of quality. Its dosage guarantees the conformity, traceability and active ingredient content of extracts for mild laxative or digestive purposes. YesWeLab offers rhapontin analysis by HPLC-DAD (high performance liquid chromatography with diode array detection) or LC-MS, depending on the nature of your samples and your analytical objectives.

What is rhapontine?

Rhapontin (CAS No. 155-58-8) is a glycosylated stilbene derivative naturally present in certain rhubarb species, notably Rheum rhaponticum . This compound is used both as a tracer of botanical authenticity and as a quality indicator in standardized plant extracts, particularly those intended for intestinal transit regulating effects.

Its presence and concentration are monitored in many rhubarb-based food supplements or herbal blends with digestive effects. It also provides evidence in the event of inspections or disputes over the botanical origin of the ingredients used.

Objectives and interests of rhapontin dosage

The dosage of rhapontin presents several interests for professionals in nutraceuticals, herbalism and phytotherapy:

• Botanical authentication: confirm the presence of rhubarb of controlled origin,
• Standardization of extracts: guarantee a constant concentration of active ingredients,
• Batch quality control: check the conformity of raw materials or finished products,
• Stability assessment: monitoring the possible degradation of active compounds over time,
• Product differentiation: promoting an ingredient through its richness in natural stilbenes.

Analytical techniques available: HPLC-DAD and LC-MS

YesWeLab offers two complementary methods for rhapontin analysis:

• HPLC-DAD: a common and robust method for quantitative analysis. It allows the detection of rhapontin with high reliability in well-characterized plant matrices, thanks to the specific UV signature of stilbene.
• LC-MS: technique of choice for complex or low-concentration matrices, with increased sensitivity. It is particularly recommended for multifractionated extracts or formulations based on plant mixtures.

Our partner laboratories validate the appropriate method based on your analytical specifications, the matrix, and the expected detection thresholds.

Matrices affected by the analysis

Rhapontin assay can be performed on a wide variety of samples:

• Dry or hydroalcoholic extracts of rhubarb,
• Rhubarb roots or powders,
• Food supplements in the form of capsules, tablets, infusions or liquids,
• Herbal blends with a mild laxative or digestive effect.

Our partner laboratories are equipped to work on complex matrices, in compliance with the quality standards of the nutraceutical industry.

Areas of application

This analysis is particularly relevant for companies in the following sectors:

• Natural food supplements,
• Phytotherapy,
• Plant extract industry,
• Formulation and quality control laboratories,
• Specialty distributors and natural health brands.

Rhapontin can also be analyzed in conjunction with other plant markers such as sennoside A, chrysin, or total flavonoids for a complete phytocompound profile. Also see our Total Flavonoid Assay or Chrysin Assay for additional analyses.

Why trust YesWeLab?

YesWeLab works directly with a network of laboratories specializing in physicochemical and nutritional analyses, selected for their expertise, responsiveness, and accreditations (ISO 17025, COFRAC, etc.). Thanks to our digital platform, you can order your analyses, track your samples, and access results with ease.

Our scientific team will help you define your needs and choose the most appropriate method, whether you are a manufacturer, formulator, or distributor of natural health products.

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Do you want to measure rhapontin in a plant-based raw material or finished product? Our team offers you a tailor-made analytical solution.