Rhapontin (stilbene) dosage

Rhapontin analysis is a reference standard for authenticating rhubarb ( Rheum spp. ) extracts used in natural food supplements. A glycosylated stilbene specific to certain rhubarb species, rhapontin is recognized as a phytological marker of quality. Its measurement ensures the conformity, traceability, and active ingredient content of extracts intended for mild laxative or digestive purposes. YesWeLab offers rhapontin analysis by HPLC-DAD (high-performance liquid chromatography with diode array detection) or LC-MS, depending on the nature of your samples and your analytical objectives.

What is rhapontine?

Rhapontin (CAS No. 155-58-8) is a glycosylated stilbene derivative naturally present in certain rhubarb species, notably Rheum rhaponticum . This compound is used both as a marker of botanical authenticity and as a quality indicator in standardized plant extracts, particularly those intended for regulating intestinal transit.

Its presence and concentration are monitored in many rhubarb-based dietary supplements or herbal blends with digestive effects. It also serves as evidence in the event of an inspection or dispute concerning the botanical origin of the ingredients used.

Objectives and benefits of rhapontin assay

The dosage of rhapontine is of interest to professionals in nutraceuticals, herbalism and phytotherapy:

• Botanical authentication: confirm the presence of rhubarb of controlled origin,
• Standardization of extracts: ensuring a constant concentration of active ingredients,
• Batch quality control: verifying the conformity of raw materials or finished products,
• Stability assessment: monitoring the potential degradation of active compounds over time,
• Product differentiation: highlighting an ingredient by its richness in natural stilbenes.

Available analytical techniques: HPLC-DAD and LC-MS

YesWeLab offers two complementary methods for rhapontin analysis:

• HPLC-DAD: a common and robust method for quantitative analysis. It allows for the highly reliable detection of rhapontin in well-characterized plant matrices, thanks to the specific UV signature of stilbene.
• LC-MS: the technique of choice for complex or low-concentration matrices, with increased sensitivity. It is particularly recommended for multi-fractionated extracts or formulations based on plant mixtures.

Our partner laboratories validate the appropriate method based on your analytical specifications, matrix, and expected detection thresholds.

Matrices involved in the analysis

Rhapontin levels can be measured on a wide variety of samples:

• Dry or hydroalcoholic extracts of rhubarb,
• Rhubarb roots or powders,
• Dietary supplements in the form of capsules, tablets, infusions or liquids,
• Herbal blends with a mild laxative or digestive effect.

Our partner laboratories are equipped to work on complex matrices, in compliance with the quality standards of the nutraceutical industry.

Areas of application

This analysis is particularly relevant for companies in the following sectors:

• Natural food supplements,
• Herbal medicine,
• Plant extracts industry,
• Formulation and quality control laboratories,
• Specialized distributors and natural health brands.

Rhapontin can also be analyzed in conjunction with other plant markers such as sennoside A, chrysin, or total flavonoids, for a complete phytocompound profile. See also our fact sheets on Total Flavonoid Assay or Chrysin Assay for further analysis.

Why trust YesWeLab?

YesWeLab works directly with a network of laboratories specializing in physicochemical and nutritional analyses, selected for their expertise, responsiveness, and accreditations (ISO 17025, COFRAC, etc.). Through our digital platform, you can easily order your analyses, track your samples, and access your results.

Our scientific team will assist you in defining your needs and choosing the most suitable method, whether you are a manufacturer, formulator, or distributor of natural health products.

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Do you want to measure rhapontine in a plant-based raw material or finished product? Our team offers you a customized analytical solution.