Pelargonidin-3,5-di-O-glucoside dosage

Pelargonidin-3,5-di-O-glucoside is an anthocyanin, a natural pigment responsible for the pinkish-red color of many fruits, flowers, and plant extracts. Derived from pelargonidin and stabilized by two glucose units, this molecule belongs to the large family of flavonoids. Its concentration allows for both the evaluation of the color intensity of an extract and the estimation of its antioxidant potential, two properties highly valued in the nutraceutical, food, and cosmetic industries.

What is pelargonidin-3,5-di-O-glucoside?
Anthocyanins are water-soluble pigments belonging to the polyphenol family, which give plants their colors ranging from red to orange. Pelargonidin-3,5-di-O-glucoside is one of the glycosylated forms of pelargonidin: the presence of two glucose groups improves its stability and solubility compared to the free aglycone. It is found notably in strawberries, pomegranates, red radishes, and various edible flowers. Like all anthocyanins, its content and stability are highly dependent on pH, temperature, light, and the extraction method, making precise quantification essential for characterizing a colored extract.

Why measure this anthocyanin?
Measuring pelargonidin-3,5-di-O-glucoside addresses several industrial challenges. For manufacturers of extracts and natural colorants, it allows them to objectively determine the color intensity of a raw material and guarantee consistency from batch to batch—a crucial criterion when color is a selling point. For nutraceuticals, the measurement serves to validate the anthocyanin content of a supplement marketed for its antioxidant properties and to support a standardization process. It also contributes to quality control by detecting pigment degradation related to storage or heat treatment. Having a reliable analytical value ensures both the visual quality and the functional value of your products.

Which analytical method?
Analysis is primarily performed using high-performance liquid chromatography with diode array detection (HPLC-DAD), which is particularly well-suited to anthocyanins that strongly absorb in the visible spectrum, allowing for selective detection and reliable quantification. For structural identification or trace analysis in complex matrices, liquid chromatography coupled with mass spectrometry (LC-MS) offers superior sensitivity and specificity. Appropriate sample preparation (extraction in acidified medium, filtration) is necessary to preserve the integrity of the pigment, which is sensitive to pH and oxidation. Our scientific team will guide you toward the most relevant method based on your matrix and objectives.

Analyzed Matrices and Related Analyses
This analysis applies to numerous matrices: fruit extracts, powders, juices, concentrates, and colored formulations. If you work with extracts rich in pigments or antioxidant compounds, also explorecolor analysis to characterize colorimetric appearance, bixin quantification (another plant pigment), or the biochemical characterization of biomolecules. Our blog articles on polyphenol and flavonoid quantificationdelve deeper into these methods. This service is relevant to the nutraceutical and plant-based.

Why choose YesWeLab?
At YesWeLab, we collaborate with a rigorously selected network of laboratories, most of which are certified and/or accredited (ISO 17025, COFRAC). These laboratories are chosen based on your specific needs, the matrices to be analyzed, and the required analytical techniques or methods. If you have any particular requirements, please do not hesitate to specify them in your request: our scientific team will do everything possible to respond accurately and promptly. Since 2020, numerous manufacturers, distributors, and engineering firms have entrusted us with the management of their analyses, sending us their samples through our digital platform. To learn more or submit a specific request, contact our team today.