Dosage of mogroside V (saponin) from grosvenori

Mogroside V is a triterpene saponin extracted from the fruit of Siraitia grosvenorii , commonly known as luo han guo or monk fruit . This compound is responsible for the fruit's intense sweetness 250 times sweeter than sucrose , yet it is calorie-free and has no impact on blood sugar levels .
Used for centuries in traditional Asian medicine, mogroside V is now a key ingredient in nutraceutical and food formulations designed to reduce sugar content without compromising flavor.

Objective of the mogroside V assay

The mogroside V assay aims to precisely quantify this active molecule in:

• Monk fruit extracts ( Siraitia grosvenorii),
• Natural sweeteners ,
• Food or nutraceutical formulations incorporating plant-based sweeteners,
• Finished products such as functional drinks, nutritional bars or slimming supplements.

This analysis ensures product standardization , consistency of sweetening power and compliance with marketing claims (“no added sugar”, “naturally sweetened”).

Analytical methods used

At YesWeLab , mogroside V is quantified by high-performance liquid chromatography (HPLC) or mass spectrometry (LC-MS/MS) . These techniques offer excellent specificity and sensitivity for the detection of this complex saponin in various matrices.

The main methods are:

• HPLC-DAD (UV/visible detection) : suitable for standardized extracts or products with high concentrations of mogroside V.
• LC-MS/MS (tandem mass spectrometry-coupled chromatography) : reference method for trace detection, identification of other mogrosides (IIE, III, IV) and confirmation of purity.

The analyses are conducted according to good laboratory practices and ISO 17025 standards , guaranteeing the reliability of the results.

Industrial applications and areas of use

The analysis of mogroside V is applicable to many industrial sectors:

• Nutraceuticals : control of the purity and dosage of natural extracts intended for food supplements.
• Functional nutrition : formulation of products with a low glycemic index , intended for diabetic consumers or those seeking natural sugar-free solutions.
• Medicinal plants : standardization of Siraitia grosvenorii extracts in the context of pharmacological studies.
• Natural cosmetics : integration of mogroside V into antioxidant or moisturizing skincare products for its softening and non-irritating effect.

Regulatory framework

In Europe, mogroside V is recognized as a natural sweetener but is not yet listed among the authorized additives (Regulation (EC) No 1333/2008). However, it is accepted in many countries (United States, Japan, Australia) as a GRAS (Generally Recognized As Safe) ingredient .

Pending full validation by the EFSA, its use in nutraceutical products and botanical extracts is regulated by the Novel Food Act (EU Regulation 2015/2283) .
Precise measurement of mogroside V is therefore essential to verify regulatory compliance and ensure transparency of composition .

Affected matrices

• Siraitia grosvenorii (monk fruit) extracts
• Blends of natural sweeteners
• Dietary supplements and sweeteners
• Beverages, bars, syrups, nutraceutical formulations
• Cosmetic products based on plant extracts

Recommended Related Analyses

For a complete characterization of natural sweetening extracts , YesWeLab recommends combining this test with:

• Assay of steviol glycoside (stevia) : monitoring of another natural sweetener with high sweetening power,
• Ginsenoside Rg1 assay : standardization of plant saponins,
• Trigonelline assay : analysis of bioactive alkaloids present in functional plants,
• Verbascoside assay : measurement of a complementary antioxidant polyphenol in plant extracts.

Why entrust your analysis to YesWeLab?

Since 2020, YesWeLab nutraceutical , food , and herbal medicine manufacturers centralize and secure their analyses through its innovative digital platform.
Our partners, accredited to ISO 17025 and often COFRAC , are selected based on the matrix, methodology, and required precision .

Through personalized scientific support, YesWeLab enables formulators, quality managers, and R&D laboratories to:

• Standardize their plant extracts,
• Verify the conformity of their products,
• Accelerate the development of new natural sweetening formulas.

Need to check the mogroside V content in your monk fruit extracts?
YesWeLab now for a quick quote and analysis compliant with international standards .