Homoisoflavone assay in ophiopogon

The quantification of homoisoflavones, specifically Ophiopogon japonicus homoisoflavones, is an essential step for those involved in nutraceuticals, phytotherapy, and plant-based cosmetics. These phenolic compounds, specific to this Asian medicinal plant, are renowned for their antioxidant, anti-inflammatory, and cell-protective effects. Their quantification allows for the standardization of plant extracts, ensures batch consistency, and validates the functional properties highlighted in finished products.

Understanding the homoisoflavones of Ophiopogon japonicus

Homoisoflavones represent a subclass of flavonoids characterized by specific, often glycosylated, chemical structures. Among the most studied in Ophiopogon japonicus are:

• Methylophiopogonanone A (CAS 74805-92-8)
• 6-Aldehydroisophiopogonone B (CAS 112500-91-1)
• Hydroxylated derivatives such as 8-hydroxylateds
• Ophiopogonone A and B

These molecules are considered specific markers of the plant and are used to validate the authenticity and quality of the extracts.

Their biological role includes:

• Protection against oxidative stress
• Potential anti-inflammatory effects
• Contributes to the protection of cells and tissues
• Possible applications in cardiovascular and immune health

Analytical methods for the determination of homoisoflavones

Homoisoflavone analysis is performed by liquid chromatography, often coupled with mass spectrometry for greater accuracy:

• HPLC (high-performance liquid chromatography) : a standardized method used to separate and quantify the main homoisoflavones in plant extracts.
• UPLC (ultra performance liquid chromatography) : an evolution of HPLC, offering finer resolution and faster analysis times.
• LC-MS or LC-MS/MS : coupling with mass spectrometry, allowing specific identification of compounds and reliable quantification even at low concentrations.

These approaches guarantee reproducible results and are suitable for complex matrices such as crude extracts, plant powders or nutraceutical formulations.

Sectors concerned by homoisoflavone analysis

The analysis of homoisoflavones in Ophiopogon japonicus is relevant in various sectors:

• Nutraceuticals : quality control of food supplements based on standardized extracts.
• Plant-based cosmetics : validation of active ingredient content for soothing and protective formulations.
• Medicinal plants : verification of phytocomplexes and standardization of extracts.
• Research and development : study of bioactive properties and exploration of new functional applications.
• Botanical biostimulants : valorizing plant extracts as innovative ingredients.

Typically analyzed matrices

Homoisoflavones are quantified in a variety of matrices:

• Plant extracts of Ophiopogon japonicus
• Standardized plant powders
• Nutraceutical capsules and tablets
• Natural serums and cosmetic products
• Traditional herbal preparations

Each matrix has analytical characteristics that require adapted and validated methods.

Quality and compliance issues

Quantifying homoisoflavones is essential to meet regulatory, commercial, and scientific expectations. The challenges include:

• Authenticity : confirming plant origin and avoiding counterfeits.
• Standardization : ensuring batch consistency and strengthening consumer confidence.
• Safety : preventing excessive variability between batches and ensuring safe formulations.
• Regulatory compliance : meeting the requirements of European and international markets.
• Marketing value : highlighting the measured and certified presence of natural assets.

YesWeLab support for your medicinal plant analyses

YesWeLab provides access to a network of partner laboratories specializing in the analysis of bioactive plant compounds. Our certified and accredited partners (ISO 17025, COFRAC) apply validated methods to guarantee reliable and internationally comparable results.

Thanks to our digital platform, you can:

• Submit your samples online with ease
• Track your analyses and get your results quickly
• Benefit from personalized scientific support

Since 2020, many manufacturers, distributors and design offices have entrusted us with their analytical projects in order to secure their developments and enhance the value of their products.

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