Ophiopogon homoisoflavones dosage

The measurement of homoisoflavones, homoisoflavones Ophiopogon japonicus, is an essential step for those involved in nutraceuticals, phytotherapy, and plant-based cosmetics. These phenolic compounds, specific to this Asian medicinal plant, are renowned for their antioxidant, anti-inflammatory, and cell-protective effects. Their quantification allows for the standardization of plant extracts, ensuring batch consistency, and validating the functional properties highlighted in finished products.

Understanding the homoisoflavones of Ophiopogon japonicus

Homoisoflavones represent a subclass of flavonoids characterized by particular chemical structures, often glycosylated. Among the most studied in Ophiopogon japonicus are:

• Methylophiopogonanone A (CAS 74805-92-8)
• 6-Aldehydoisoophiopogonone B (CAS 112500-91-1)
• Hydroxy derivatives such as 8-hydroxylated
• Ophiopogonone A and B

These molecules are considered specific markers of the plant and are used to validate the authenticity and quality of the extracts.

Their biological role includes:

• Protection against oxidative stress
• Potential anti-inflammatory effects
• Contribution to the protection of cells and tissues
• Possible applications in cardiovascular and immune well-being

Analytical methods for the determination of homoisoflavones

Homoisoflavones analysis is performed by liquid chromatography, often coupled with mass spectrometry for greater precision:

• HPLC (high-performance liquid chromatography) : a standardized method used to separate and quantify the main homoisoflavones in plant extracts.
• UPLC (ultra performance liquid chromatography) : an evolution of HPLC, offering finer resolution and faster analysis times.
• LC-MS or LC-MS/MS : coupling with mass spectrometry, allowing specific identification of compounds and reliable quantification even at low concentrations.

These approaches guarantee reproducible results adapted to complex matrices such as crude extracts, plant powders or nutraceutical formulations.

Sectors concerned by the analysis of homoisoflavones

The analysis of homoisoflavones from Ophiopogon japonicus is relevant in different sectors:

• Nutraceutical : quality control of food supplements based on standardized extracts.
• Plant-based cosmetics : validation of the active ingredient content for soothing and protective formulations.
• Medicinal plants : verification of phytocomplexes and standardization of extracts.
• Research and development : study of bioactive properties and exploration of new functional applications.
• Botanical biostimulants : valorization of plant extracts as innovative ingredients.

Typically analyzed matrices

Homoisoflavones are quantified in a variety of matrices:

• Plant extracts of Ophiopogon japonicus
• Standardized plant powders
• Nutraceutical capsules and tablets
• Serums and natural cosmetic products
• Traditional herbal medicine preparations

Each matrix has analytical characteristics that require adapted and validated methods.

Quality and compliance issues

Quantification of homoisoflavones is essential to meet regulatory, commercial, and scientific expectations. Challenges include:

• Authenticity : confirm plant origin and avoid counterfeits.
• Standardization : ensuring batch consistency and building consumer confidence.
• Safety : Prevent excessive batch-to-batch variability and ensure safe formulations.
• Regulatory compliance : meeting the requirements of European and international markets.
• Marketing promotion : highlight the measured and certified presence of natural active ingredients.

YesWeLab support for your medicinal plant analyses

YesWeLab provides a network of partner laboratories specializing in the analysis of bioactive plant compounds. Our partners, certified and accredited (ISO 17025, COFRAC), apply validated methods to guarantee reliable and internationally comparable results.

Thanks to our digital platform, you can:

• Submit your samples online with ease
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Since 2020, many manufacturers, distributors and design offices have entrusted us with their analytical projects in order to secure their developments and enhance the value of their products.

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