Dosage of chrysophanol (anthraquinone / hydroxyanthracene)

Chrysophanol (CAS No. 481-74-3) is a natural anthraquinone , also known as 1,8-dihydroxy-3-methylanthraquinone medicinal plants such as rhubarb (Rheum palmatum) , senna (Cassia angustifolia), and aloe . This compound belongs to the hydroxyanthracenes , molecules known for their laxative and anti-inflammatory effects , but also monitored for their potential health risks at high doses .

The dosage of chrysophanol makes it possible to control the concentration of hydroxyanthracenes in plant extracts, powders and food supplements , in order to guarantee regulatory compliance and the safety of products intended for human consumption.

Analytical methods: HPLC and LC-MS

The analysis of chrysophanol relies on high-precision chromatographic techniques:

• HPLC (high-performance liquid chromatography) : reference method for separating and quantifying anthraquinones in complex extracts.
• LC-MS (liquid chromatography coupled with mass spectrometry) : offers high molecular specificity and ultra-sensitive detection , ideal for confirming the presence of chrysophanol and its derivatives.

These methods comply with the analytical recommendations of the European Pharmacopoeia reliable and reproducible results even at very low concentrations.

Industrial and regulatory applications

Chrysophanol dosage is essential in the following areas:

• Nutraceutical : standardization of extracts of rhubarb, senna, aloe or polygonum.
• Phytotherapy : control of anthraquinone content for the safety of products intended for digestive use.
• Regulation : verification of compliance with Regulation (EU) 2021/468 , prohibiting the use of certain substances derived from hydroxyanthracene (including chrysophanol at certain concentrations).
• Research and development : characterization of new plant sources and study of the stability of anthraquinones.

This service can be complemented by the measurement of emodin and rhein , two other anthraquinones commonly present in the same plant extracts.

Affected matrices

Our partner laboratories perform chrysophanol assays on various matrices:

• Dry or liquid extracts of medicinal plants (rhubarb, senna, aloe, polygonum)
• Food supplements (capsules, powders, tablets)
• Plant raw materials
• Nutraceutical or phytotherapeutic finished products

Each method is adapted to the analysis matrix and the applicable regulatory threshold , guaranteeing traceability and conformity of results.

YesWeLab expertise

At YesWeLab , we collaborate with a network of over 200 partner laboratories in France, Europe and internationally , rigorously selected for their expertise in phytochemical analyses , their state-of-the-art chromatographic equipment , and their ISO 17025 and COFRAC accreditations .

Our scientific team supports you at every stage: method selection , referral to the most suitable laboratory , and complete tracking of your samples via our digital platform . This allows you to centralize your requests , obtain a quick quote , and access your results online .

Since 2020, many nutraceutical manufacturers, plant extract laboratories and formulators have trusted us for their anthraquinone and hydroxyanthracene compliance checks .

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