Dosage of beta-carboline (indole alkaloid MTCA)

Beta-carbolines are a family of indole alkaloids found naturally in certain medicinal plants, fermented foods, and alcoholic beverages. Among them, 1-methyltetrahydrobeta-carboline-3-carboxylic acid (MTCA) is a bioactive molecule that is attracting increasing interest in nutraceutical, toxicological, and neuropharmacological research.

The MTCA beta-carboline assay allows for the identification and quantification of this compound at fine concentrations, whether in plant extracts, fermented matrices or nutritional formulations. This is an essential analysis for:

• Evaluate the quality or safety of plant extracts
• Study the neuroactive profile of certain foods or drinks
• Control the presence of potentially bioactive or toxic metabolites
• Conduct pharmacokinetic or chronic exposure studies

Available analytical methods

Tandem mass spectrometry (LC-MS/MS) is the gold standard for the determination of MTCA and related beta-carbolines. It enables specific detection and precise quantification, even at trace levels, in complex matrices. High-performance liquid chromatography (HPLC) coupled with UV or MS detection can also be used for targeted analyses, particularly in routine quality control.

The selection of the method depends on:

• of the type of matrix (plant extract, drink, fermented, supplement)
• of the expected sensitivity
• the presence of potential interferents
• regulatory or scientific objectives (toxicity, bioactivity, traceability)

Affected matrices

Beta-carboline analysis can be applied to a wide variety of products, including:

• Botanical extracts (Peganum harmala, Banisteriopsis caapi, etc.)
• Fermented foods (soy sauces, aged cheeses, wines)
• Alcoholic or fermented beverages
• Nutritional and herbal supplements

Industrial and scientific applications

The dosage of MTCA beta-carboline meets the needs:

• Food supplement manufacturers seeking to control the quality and compliance of their extracts
• Research laboratories studying the neuroactive or psychoactive impact of food alkaloids
• Food industry manufacturers keen to evaluate secondary compounds resulting from fermentation or thermal interactions
• Food safety and toxicology specialists are seeking data on chronic exposure to these compounds.

Why analyze MTCA with YesWeLab?

YesWeLab connects you with specialized laboratories equipped for the ultra-sensitive analysis of plant alkaloids and metabolic tracers such as MTCA. Our partners, most of whom are ISO 17025 or COFRAC accredited, are experts in chromatography and spectrometry protocols adapted to regulatory and scientific requirements.

Our team supports you every step of the way:

• Definition of the analytical need
• Selecting the appropriate method
• Processing and logistical monitoring of samples
• Delivery of usable, validated and commented results

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To enrich your analyses and complete your analytical strategy, you can consult:

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Since 2020 , YesWeLab has facilitated access to complex analytical services thanks to its digital platform and network of partner laboratories. Submit your sample today via our online interface or contact our experts for personalized support.