Dosage of beta-carboline (indole alkaloid MTCA)

Beta-carbolines are a family of indole alkaloids found naturally in certain medicinal plants, fermented foods, and alcoholic beverages. Among them, 1-methyl-tetrahydro-beta-carboline-3-carboxylic acid (MTCA) is a bioactive molecule that is attracting increasing interest in nutraceutical, toxicological, and neuropharmacological research.

The measurement of beta-carboline MTCA allows for the identification and quantification of this compound at low concentrations, whether in plant extracts, fermented matrices, or nutritional formulations. This analysis is essential for:

• Evaluate the quality or safety of plant extracts
• Studying the neuroactive profile of certain foods or drinks
• Monitor for the presence of potentially bioactive or toxic metabolites
• Conducting pharmacokinetic or chronic exposure studies

Available analytical methods

Tandem mass spectrometry (LC-MS/MS) is the reference method for the determination of MTCA and related beta-carbolines. It allows for specific detection and precise quantification, even at trace levels, in complex matrices. High-performance liquid chromatography (HPLC) coupled with UV or MS detection can also be used for targeted analyses, particularly in routine quality control.

The choice of method depends on:

• of the matrix type (plant extract, beverage, fermented, supplement)
• of the expected sensitivity
• the presence of potential interferers
• regulatory or scientific objectives (toxicity, bioactivity, traceability)

Affected matrices

Beta-carboline analysis can be applied to a wide variety of products, including:

• Botanical extracts (Peganum harmala, Banisteriopsis caapi, etc.)
• Fermented foods (soy sauces, aged cheeses, wines)
• Alcoholic or fermented beverages
• Nutritional and phytotherapeutic supplements

Industrial and scientific applications

The MTCA beta-carboline assay meets the following requirements:

• Dietary supplement manufacturers seeking to control the quality and compliance of their extracts
• Research laboratories studying the neuroactive or psychoactive impact of food alkaloids
• Food industry professionals are keen to evaluate secondary compounds resulting from fermentation or thermal interactions
• Food safety or toxicology specialists seeking data on chronic exposure to these compounds

Why analyze MTCA with YesWeLab?

YesWeLab connects you with specialized laboratories equipped for the ultra-sensitive analysis of plant alkaloids and metabolic tracers such as MTCA. Our partners, most of whom are ISO 17025 or COFRAC accredited, are experts in chromatography and spectrometry protocols adapted to regulatory and scientific requirements.

Our team will support you every step of the way:

• Definition of the analytical need
• Selection of the appropriate method
• Sample processing and logistical tracking
• Delivery of usable, validated and commented results

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Since 2020 , YesWeLab has facilitated access to complex analytical services through its digital platform and network of partner laboratories. Submit your sample today via our online interface or contact our experts for personalized support.