Dosage of coumestrol (coumestan)

Coumestrol (CAS No. 479-13-0) is a natural phytoestrogen belonging to the coumestan family. It is found in several leguminous plants such as soybeans, clover, and alfalfa sprouts. Due to its chemical structure, which is similar to estrogens, it exhibits moderate hormonal activity, making it a molecule of interest for phytotherapy and functional nutrition.

The dosage of coumestrol allows for the precise quantification of its concentration in plant extracts, powders, functional foods or food supplements, in order to guarantee the quality, traceability and regulatory compliance of the products.

Analytical methods: HPLC and LC-MS

The analysis of coumestrol relies on high-precision chromatographic techniques:

• HPLC (high-performance liquid chromatography): a method commonly used to separate and quantify phytoestrogens in complex matrices of plant origin.
• LC-MS (liquid chromatography coupled with mass spectrometry): offers high molecular specificity and sensitivity, allowing the detection of coumestrol in trace amounts.

These methods ensure reliable, reproducible results that comply with international analytical standards (ISO, AOAC, EFSA).

Industrial and scientific applications

The dosage of coumestrol is applicable to several areas:

• Nutraceuticals and food supplements: standardization of plant extracts rich in phytoestrogens.
• Phytotherapy: evaluation of the natural hormonal potential of certain medicinal plants.
• Nutrition research: study of the effects of coumestrol on metabolic and hormonal health.
• Agronomy and plant breeding: monitoring phytoestrogen levels in legume crops.

This service can be complemented by the measurement of isoflavones (genistein, daidzein, formononetin) or resveratrol , in order to obtain a complete characterization of bioactive phenolic compounds.

Affected matrices

Our partner laboratories perform coumestrol assays on various types of matrices:

• Plant extracts (soybean, clover, alfalfa, sprouts, red clover)
• Nutraceutical powders and ingredients
• Finished products (food supplements, capsules, functional drinks)
• Plant-based raw materials and R&D formulations

Each protocol is adapted to the original matrix, the expected content and the client's analytical objectives.

YesWeLab expertise

At YesWeLab , we collaborate with a network of more than 200 partner laboratories located in France, Europe and internationally, rigorously selected for their technical expertise, their ISO 17025 and COFRAC accreditations, and their mastery of phytochemical analyses.

Our scientific team will assist you in choosing the analytical protocol, selecting the appropriate laboratory, and monitoring your analyses via our digital platform. This platform allows you to centralize your requests, obtain quick quotes, and track your samples in real time.

Since 2020, many nutraceutical manufacturers, formulators and distributors have trusted us to ensure the quality, compliance and traceability of their plant extracts.

Order a coumestrol (coumestan) analysis

Do you want to quantify coumestrol in your plant extracts, functional foods, or nutraceutical products?
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