FTIR microscopy: for localized chemical analysis of micro-contaminants and defects
FTIR microscopy combines the principles of Fourier transform infrared spectroscopy with optical imaging, enabling the localization and chemical identification of microscopic areas of a sample . This non-destructive method is ideal for the analysis of micro-contaminants, localized defects, inclusions or thin layers in complex matrices. It is widely used in the materials, medical, cosmetics, packaging and pharmaceutical sectors.
What is FTIR microscopy?
FTIR microscopy ( or FTIR microspectroscopy) is based on the integration of an optical microscope with an FTIR spectrometer . It allows a very precise area of the sample (down to a few micrometers) to be visually targeted and the infrared spectrum . This spectrum reveals the chemical composition of the analyzed point, thus allowing the identification of contaminants, residues, degradations or specific layers.
This technique is particularly suited to the study of local heterogeneities invisible to the naked eye, or to the characterization of very small quantities of matter .
How FTIR microscopy works
An FTIR microscopy system is composed of:
- Optical microscope : allows precise visualization of the sample and selection of the area to be analyzed.
- FTIR System : Records the IR spectrum of the targeted area.
- Specific IR lenses : ensure the focusing of the IR beam on a micrometric surface.
- Analysis modes possible:
- Transmission : for thin or pellet samples
- Reflection (IRRAS, DRIFTS) : for flat or metallic surfaces
- ATR (Attenuated Total Reflectance) : for very localized areas or particles
Technical characteristics of FTIR microscopy
- Spatial resolution : up to 10 µm
- Non-destructive method
- Precise chemical identification on localized areas
- Compatible with complex or multi-layer samples
- Measurement possible on micro-fragments, inclusions, deposits, fibers, etc.
- Spectra comparable to FTIR databases
- Applicable standards according to sectors : ISO 16000-6, ICH Q2(R1), ISO 21461, REACH, pharmacopoeias, etc.
For which matrices?
FTIR microscopy is particularly suitable for or semi-solid samples . It is ideal for:
Main industrial applications
LC-MS and LC-MS/MS are widely used in R&D, method validation, quality control and regulatory compliance , particularly in the following sectors:
Léa Géréec
Technical and scientific advisor
