Yohimbine Dosage

Yohimbine testing allows for the quantification of this natural alkaloid extracted from the bark of Pausinystalia yohimbe , a tree native to West Africa. Long used for its vasodilatory and libido-enhancing effects, yohimbine is now regulated in several countries due to its significant pharmacological activity. Therefore, its measurement is essential to ensure the compliance of dietary supplements, plant extracts, and aphrodisiac formulations containing this molecule.

The analysis is carried out by high-performance liquid chromatography (HPLC) or liquid chromatography coupled with mass spectrometry (LC-MS/MS), two reference methods for the detection and quantification of alkaloids at low concentrations.

Principle and operation of the method

The sample (yohimbe extract, powder, capsule, tablet or complex formulation) is prepared by selective extraction of alkaloids using a suitable solvent.

• In HPLC-DAD, separation is based on the polarity and molecular size of the compounds, with detection achieved by UV-visible absorption.
• In LC-MS/MS, yohimbine is identified according to its mass/charge ratio (m/z), offering exceptional specificity and sensitivity, even in trace amounts.

These methods guarantee the reliability and regulatory compliance of marketed products, while also allowing for labeling verification and the detection of potential fraud or overdosing.

Analytical performance and related analyses

HPLC and LC-MS/MS chromatographic methods offer:

• high sensitivity (detection at a few ng/mL),
• excellent reproducibility,
• and a high selectivity towards complex plant matrices.

For a complete characterization of alkaloid-containing extracts, this analysis can be combined with:

• the dosage of oxindolic alkaloids ,
• the determination of total alkaloids,
• flavonoid dosage
• or the measurement of total polyphenols

These additional services make it possible to characterize the complete phytochemical profile of plant extracts used in nutraceuticals.

Applications and areas of use

Yohimbine testing is primarily used in the following sectors:

• Nutraceuticals : verification of the actual yohimbine content in food supplements and standardized extracts.
• Phytotherapy : control of the conformity of extracts of Pausinystalia yohimbe used for tonic or aphrodisiac purposes.
• Regulatory control : assessment of the conformity of imported or marketed products, in order to comply with legal concentration thresholds.
• Research and development : studies on the formulation and efficacy of natural products containing bioactive alkaloids.

Adherence to regulated concentrations is essential, as excessively high levels can cause adverse effects (hypertension, tachycardia, anxiety).

The advantages of the YesWeLab service

YesWeLab relies on a network of partner laboratories specializing in phytochemical analyses , accredited to ISO 17025 and COFRAC standards. Each project is assigned to the most suitable laboratory based on the matrix, method, and applicable regulations.

Thanks to its digital analysis platform, YesWeLab simplifies the management of your services: requesting quotes, sending samples, and receiving results online. Since 2020, numerous players in the nutraceutical, phytotherapy, and plant R&D sectors have trusted YesWeLab for their alkaloid and bioactive compound analyses.

For any specific yohimbine assay needs, contact our scientific team to obtain a personalized quote and analytical support tailored to your needs.