Xanthotoxin (furanocoumarin) assay

Xanthotoxin , also known as 8-methoxypsoralen, is a furanocoumarin naturally present in certain plants of the Apiaceae and Rutaceae families, such as celery, parsley, fig, and bergamot. This molecule is known for its potent photosensitizing effects, and its accumulation can lead to skin reactions in the presence of UV light.

While used in low doses in dermatology (particularly in PUVA therapy), xanthotoxin is strictly regulated in cosmetic products, foodstuffs, and plant extracts. Its dosage is essential to guarantee consumer safety and ensure compliance with European regulations (Regulation (EC) No 1223/2009, SCCS recommendations).

Analytical methods: HPLC-DAD and LC-MS

Quantitative analysis of xanthotoxin relies on liquid chromatography techniques adapted to complex extracts:

• HPLC-DAD (diode array detection): a common and effective method for the determination of xanthotoxin in essential oils, extracts or cosmetics.
• LC-MS (liquid chromatography coupled with mass spectrometry): recommended for matrices with low concentration or for further research into associated furanocoumarins (bergapten, isopimpinellin, etc.).

The methods used in our network of partner laboratories allow for precise and sensitive quantification, in accordance with current standards, and applicable to different types of products.

Why measure xanthotoxin levels?

Xanthotoxin measurement addresses several industrial, regulatory, and health-related challenges:

• Ensuring the safe use of plant extracts in cosmetic or food products.
• Respect the maximum levels allowed by European cosmetic regulations (≤ 1 mg/kg in leave-on products containing photosensitizing essential oils).
• Controlling natural extracts from plants rich in furanocoumarins (celery, angelica, bergamot, etc.).
• Identify potential risks of photosensitivity or toxicity through ingestion or skin contact.
• Meeting the requirements of quality certifications (COSMOS, ISO 16128, etc.) or exports.

Matrices analyzed

Our partner laboratories perform xanthotoxin assays in various matrices:

• Essential oils (bergamot, lemon, angelica, etc.)
• Plant extracts for food supplements or cosmetics
• Cosmetics: creams, lotions, perfumes, sunscreens
• Foods: celery juice, marinades, jams, flavored preparations
• Technical formulations: premix, botanical raw materials, incense

Each matrix is ​​prepared according to a rigorous protocol (extraction, filtration, purification) to ensure reliable quantification of xanthotoxin.

Areas of application

Xanthotoxin measurement is essential in several industrial sectors:

• Cosmetics industry: control of photosensitizing essential oils, compliance of leave-on products
• Nutraceuticals and phytotherapy: safety of plant extracts rich in furanocoumarins
• Food industry: control of juices, drinks and preparations containing celery, fennel or bergamot
• Analytical research: studies on skin toxicity, UV/plant molecule interactions

Accredited laboratories, furanocoumarin expertise

YesWeLab relies on a network of laboratories specializing in the analysis of hazardous plant compounds, particularly furanocoumarins. These partners are ISO 17025 certified and use validated methods adapted to the requirements of European and international authorities.

We support you in defining your analytical thresholds, interpreting the results and ensuring the regulatory compliance of your products.

A digital platform to manage your analyses

Since 2020, YesWeLab has provided you with an intuitive digital platform, allowing you to:

• Submit your analysis requests in just a few clicks
• Track the progress of your analytical projects
• Download your analysis reports and certificates
• Engage with our dedicated scientific team

We are the leading analytical partner for manufacturers of plant extracts, natural cosmetics, food supplements and agri-food products.

Further analyses recommended

• In vitro phototoxicity test (OECD 432)

Add the xanthotoxin dosage to your quote

Do you want to monitor the xanthotoxin content of your extracts, oils, or finished products? Describe your needs to our scientific team and benefit from responsive, expert support. YesWeLab, your analytical solution for compliance and safety of natural products.