Vitexin (flavone) dosage

Vitexin is a C-glycosylated flavone naturally present in many medicinal plants such as passionflower (Passiflora incarnata), fenugreek (Trigonella foenum-graecum), and hawthorn. It belongs to the flavonoid family, known for their antioxidant, anti-inflammatory, and neuroprotective properties. Used in dietary supplements for their calming, cardiotonic, or vasoregulatory effects, vitexin is currently attracting increasing interest in nutraceuticals.

Vitexin testing ensures the quality, concentration, and conformity of plant extracts used in natural health products. It is essential for standardizing formulations and guaranteeing their effectiveness.

Analytical methods: HPLC-DAD and LC-MS

Quantitative analysis of vitexin is performed by high-performance liquid chromatography, using two complementary techniques:

• HPLC-DAD (diode array detection): a robust and common method for quantifying vitexin in plant extracts or standardized powders.
• LC-MS (liquid chromatography coupled with mass spectrometry): recommended for complex or low-content matrices, as well as for the search for possible conjugated forms or impurities.

Our partner laboratories apply validated methods that comply with international standards (ISO 17025, ICH, Ph. Eur.), guaranteeing reliable and reproducible results, even on multicomponent extracts.

Why measure vitexin levels?

Vitexin measurement is part of a quality control, product enhancement, and regulatory compliance process. It allows, in particular, for:

• Standardize plant extracts for nutraceutical or phytotherapeutic purposes.
• Check the functional activity (antioxidant, soothing, hypotensive) claimed by the manufacturers.
• Control production batches to ensure the homogeneity of formulations.
• To meet the requirements of health authorities for claims or exports to third countries.
• Detect alterations or loss of activity during storage, drying or encapsulation.

Analyzed matrices

Vitexin can be dosed in a wide variety of products and pharmaceutical forms:

• Dry plant extracts (passionflower, hawthorn, fenugreek, etc.)
• Micronized plant powders
• Dietary supplements: capsules, tablets, herbal teas in sachets, liquid extracts
• Technical formulations: phytoconstituent complexes, premixes, plant-based syrups

Each matrix is ​​prepared according to a suitable protocol in order to maximize the accuracy of the analysis and the recovery of the target molecule.

Areas of application

Vitexin measurement is essential for several segments of the nutraceutical and herbal products industry:

• Calming or mental well-being dietary supplements
• Products for cardiovascular, hypotensive or circulatory purposes
• Antioxidant and protective formulations against oxidative stress
• Traditional products or products derived from Chinese, Ayurvedic or European pharmacopoeia
• Research on polyphenols and flavones of pharmacological interest

Accredited laboratories and plant expertise

At YesWeLab, we work exclusively with laboratories selected for their expertise in phytochemistry, their state-of-the-art equipment (HPLC, LC-MS/MS, UV-DAD) and their ISO 17025 accreditation. We offer you tailored support, whether for R&D projects, routine analyses, batch controls or regulatory dossiers.

Our partners have mastered the specific constraints related to complex extracts, variable content of active ingredients and regulatory requirements of different markets.

Simplified management with our digital platform

Since 2020, YesWeLab has been supporting professionals in the agri-food, natural health, and dietary supplement sectors through its intuitive digital platform, which enables:

• To submit your analysis requests online
• To track the progress of your cases in real time
• To view and archive your analysis reports
• To benefit from dedicated scientific support

Each project receives personalized support from our team of experts.

Further analyses recommended

• Total flavonoid content assay

Get your personalized quote now

Add the vitexin dosage to your quote request or contact our scientific team. YesWeLab supports you to guarantee the quality and compliance of your flavone-based extracts.