Vitamin K dosage

Vitamin K is a fat-soluble vitamin essential for the proper functioning of the body, particularly in blood clotting, bone mineralization, and the regulation of calcium metabolism. It exists in two main forms:

• Vitamin K1 (phylloquinone): of plant origin, mainly found in green leafy vegetables and oils.
• Vitamin K2 (menaquinones): of bacterial origin, found in some fermented products or of animal origin, in different forms (MK-4 to MK-13).

Vitamin K dosage is essential for manufacturers developing bone health products, cardiovascular food supplements or enriched formulations, but also for manufacturers of functional foods or animal nutrition.

Analytical method: LC-MS/MS (tandem mass spectrometry)

Vitamin K is primarily analyzed by LC-MS/MS , a reference method allowing excellent specificity and reliable distinction between K1 and K2 , including in complex matrices.

This technique allows you to:

• Measuring alpha-phylloquinone (K1)
• Identify and quantify the different forms of menaquinones (K2), such as MK-4, MK-7, etc.
• Achieving very low detection limits, suitable for low-dose products or complex matrices
• Perform comprehensive vitamin profiles if necessary (upon request)

Our partner laboratories validate the methods according to international standards (ISO 17025, USP, Ph. Eur.) to guarantee the reliability and reproducibility of the results.

Why measure vitamin K levels?

Vitamin K testing addresses quality, health, and regulatory issues:

• Quality control of enriched raw materials (plant extracts, oils, premixes)
• Validation of health claims authorized by EFSA or other regulatory authorities
• Formulation and verification of dosages in food supplements or fortified foods
• Monitoring stability in sensitive formulations (lipids, emulsions, multivitamin tablets)
• Differentiation of K1/K2 forms for products specifically targeting bone or vascular health

Affected matrices

Vitamin K can be measured in a wide variety of matrices using appropriate protocols:

• Dietary supplements: tablets, capsules, ampoules, oils, powders
• Enriched vegetable or animal oils
• Food products: infant formula, margarine, nutritional bars, fermented foods
• Plant extracts rich in phylloquinone
• Raw materials or technical ingredients
• Premixes and animal nutrition products

Areas of application

Vitamin K testing is relevant for many industrial sectors:

• Nutraceutical industry: products targeting bone density, circulation, and vitamin K2 deficiencies
• Cosmetics (more marginal): for certain targeted treatments (e.g., concealers, vascular products)
• Agri-food: development of fortified functional foods
• Animal nutrition: formulation of specific feeds for species with K1 or K3 requirements
• Clinical or academic research: study of bioavailability, comparative K1/K2 effects, or metabolism

Specialized and accredited laboratories

YesWeLab collaborates exclusively with ISO 17025 accredited laboratories specializing in nutritional and physicochemical analyses. Depending on your needs, we will direct you to:

• The appropriate dosage type (K1 only, specific K2, or full profile)
• The optimal method (LC-MS/MS or HPLC in some cases)
• The laboratory with the sector-specific expertise related to your product

All services are performed in compliance with good analytical practices, with clear, usable results accompanied, if necessary, by a scientific interpretation.

A digital platform to simplify your requests

Since 2020, YesWeLab has simplified the management of laboratory analyses through its online platform . In just a few clicks, you can:

• Add the vitamin K dosage to your quote request
• Track the status of your samples
• Access your secure analysis reports
• Discuss your needs with our scientific team to refine them

We provide personalized support at every stage of the project.

Further analyses recommended

• Vitamin A dosage
• Vitamin D dosage
• Vitamin E dosage

Request a personalized quote now

Add this service to your online cart or contact our scientific team. YesWeLab provides rigorous and responsive support for all your vitamin K and enriched formulation analyses.