Vitamin K dosage

Vitamin K is a fat-soluble vitamin essential for the proper functioning of the body, particularly in the processes of blood clotting, bone mineralization and regulation of calcium metabolism. It comes in two main forms:

• Vitamin K1 (phylloquinone): plant-based, found mainly in green leafy vegetables and oils.
• Vitamin K2 (menaquinones): of bacterial origin, found in certain fermented or animal products, in different forms (MK-4 to MK-13).

Vitamin K dosage is essential for manufacturers developing bone health products, cardiovascular food supplements or enriched formulations, but also for manufacturers of functional foods or animal nutrition.

Analytical method: LC-MS/MS (tandem mass spectrometry)

Vitamin K is analyzed mainly by LC-MS/MS , a reference method allowing excellent specificity and reliable distinction between K1 and K2 , including in complex matrices.

This technique allows you to:

• Dose alpha-phylloquinone (K1)
• Identify and quantify the different forms of menaquinones (K2), such as MK-4, MK-7, etc.
• Achieve very low detection limits, suitable for low-dose products or complex matrices
• Carry out complete vitamin profiles if necessary (on request)

Our partner laboratories validate the methods according to international standards (ISO 17025, USP, Ph. Eur.) to guarantee the reliability and reproducibility of the results.

Why measure vitamin K?

The dosage of vitamin K responds to quality, health and regulatory issues:

• Quality control of enriched raw materials (plant extracts, oils, premix)
• Validation of health claims authorized by EFSA or other regulatory authorities
• Formulation and verification of doses in food supplements or fortified foods
• Stability monitoring in sensitive formulations (lipids, emulsions, multivitamin tablets)
• Differentiation of K1/K2 forms for products specifically targeting bone or vascular health

Affected matrices

Vitamin K can be measured in a large number of matrices using suitable protocols:

• Food supplements: tablets, capsules, ampoules, oils, powders
• Enriched vegetable or animal oils
• Food products: infant milk, margarines, nutritional bars, fermented foods
• Plant extracts rich in phylloquinone
• Raw materials or technical ingredients
• Premix and animal nutrition products

Areas of application

Vitamin K dosage is relevant for many industrial sectors:

• Nutraceutical industry: products targeting bone density, circulation, vitamin K2 deficiency
• Cosmetics (more marginal): for certain targeted treatments (e.g.: concealer, vascular products)
• Agri-food: development of enriched functional foods
• Animal nutrition: formulation of specific feeds for species with K1 or K3 requirements
• Clinical or academic research: study of bioavailability, comparative K1/K2 effects, or metabolism

Specialized and accredited laboratories

YesWeLab works exclusively with ISO 17025-accredited laboratories specializing in nutritional and physicochemical analyses. Depending on your needs, we can direct you to:

• The type of dosage adapted (K1 alone, specific K2, or complete profile)
• The optimal method (LC-MS/MS or HPLC for certain cases)
• The laboratory with the sector expertise related to your product

All services are carried out in compliance with good analytical practices, with clear, usable results, accompanied if necessary by scientific interpretation.

A digital platform to simplify your requests

Since 2020, YesWeLab has made it easier to manage laboratory analyses thanks to its online platform . In just a few clicks, you can:

• Add vitamin K dosage to your quote request
• Track the status of your samples
• Access your secure analysis reports
• Talk to our scientific team to refine your needs

We provide personalized support to you at every stage of the project.

Further analyses recommended

• Vitamin A dosage
• Vitamin D dosage
• Vitamin E dosage

Request a personalized quote now

Add this service to your online cart or contact our scientific team. YesWeLab provides rigorous and responsive support for all your vitamin K and enriched formulation analyses.