Vitamin C dosage

Vitamin C, or ascorbic acid, is a water-soluble vitamin essential for the proper functioning of the body. A major antioxidant, it plays a role in collagen synthesis, cell protection against oxidative stress, iron absorption, and the proper functioning of the immune system. Highly sensitive to heat, light, and oxygen, its actual content in finished products can vary considerably. Therefore, its measurement is essential to validate the composition of plant extracts, dietary supplements, cosmetics, and fortified fresh produce.

Analytical methods: HPLC-DAD and colorimetric titration

YesWeLab offers two reference methods for vitamin C analysis:

• HPLC-DAD (high-performance liquid chromatography with UV-visible detection): the method of choice for accurately quantifying ascorbic acid in complex or sensitive matrices. It also allows for the differentiation of ascorbic acid from its oxidized (dehydroascorbic) form.
• Titrimetry (2,6-dichlorophenolindophenol method): a rapid and commonly used method for simple matrices (juices, fresh fruits, solutions) or routine controls. It is based on the redox reaction between vitamin C and a specific dye.

Depending on the nature of your samples and the expected requirements (accuracy, regulations, inter-batch comparison, etc.), we will guide you towards the most suitable method.

Why measure vitamin C levels?

Vitamin C dosage helps to meet several critical needs:

• Validate the enrichment of food or nutraceutical products in accordance with the authorized claims (source of vitamin C, helps reduce fatigue, supports the immune system…).
• Check the stability of ascorbic acid in formulations sensitive to oxidation or storage (juices, powders, extracts).
• Control the quality of plant-based raw materials, particularly plant extracts rich in natural vitamin C (acerola, rosehip, camu-camu, etc.).
• Documenting the compliance of cosmetic products claiming antioxidant, anti-aging or skin radiance action.
• Meeting regulatory requirements in the formulation of supplements or fortified foods.

Analyzed matrices

Vitamin C can be measured in a wide variety of matrices, including:

• Food products: fruit juices, fortified drinks, purees, baby food
• Dietary supplements: tablets, capsules, powders, sachets
• Plant extracts standardized in vitamin C (acerola, amla, camu-camu, etc.)
• Cosmetics: serums, lotions, antioxidant creams
• Raw materials or technical ingredients (pure vitamin C, additives)

Each sample is prepared according to a suitable protocol, in order to efficiently extract ascorbic acid without altering it.

Sectors of application

Vitamin C dosage is essential for many industrial players:

• Food industry: formulation and control of fortified foods, fresh juices or processed products
• Nutraceuticals: development of targeted supplements for immunity, energy, skin, or antioxidants
• Cosmetics: Evaluation of the actual concentration of vitamin C in facial or anti-aging skincare products
• Animal nutrition: formulation of foods enriched with vitamin C for animals unable to synthesize it (e.g., guinea pigs, fish)
• Research and development: stability monitoring, bioavailability studies, or comparative analysis between batches

Accredited laboratories for your vitamin analyses

YesWeLab works with ISO 17025 accredited laboratories specializing in physicochemical and nutritional analyses. Depending on your needs, we will direct you to the most competent laboratory for your chosen method (HPLC or titration), taking into account the nature of your samples, your quality objectives, and regulatory requirements.

Simplified management via our digital platform

Since 2020, YesWeLab has been supporting food, cosmetics, and healthcare manufacturers with their analytical needs. Through our online platform, you can:

• Request a personalized quote
• Track your samples in real time
• Access your analysis reports
• Ask your questions to our scientific team

Our goal: to offer you a rigorous, transparent and responsive service to ensure the reliability of your products at every stage.

Further analyses recommended

• Vitamin B1 dosage
• Vitamin B2 dosage
• Vitamin B6 dosage
• Vitamin B12 dosage
• Vitamin B8 dosage

Get a personalized quote

Add this service to your online request or contact our scientific team. YesWeLab supports your vitamin analyses with precision, speed, and expertise, regardless of your industry.