Vitamin B2 (riboflavin) dosage

Riboflavin , better known as vitamin B2 , is a measurement method for quantifying this essential water-soluble vitamin in various food products, supplements, and nutraceutical formulations. Vitamin B2 is involved in numerous enzymatic reactions and plays a central role in cellular energy production, amino acid, lipid, and carbohydrate metabolism, and in maintaining healthy skin and mucous membranes. Its measurement is crucial for manufacturers wishing to guarantee nutritional intake, justify a claim, or enrich a plant or animal matrix.

Analytical methods: HPLC-DAD, LC-MS and fluorescence

YesWeLab offers several reference techniques for riboflavin analysis, depending on the nature of the matrix and the required precision:

• HPLC-DAD (high performance liquid chromatography with UV-DAD detection): classic and reliable method for standard food products and extracts.
• LC-MS (liquid chromatography coupled with mass spectrometry): particularly suitable for complex matrices or trace measurements.
• Fluorescence after derivatization: highly sensitive method, used in particular for beverages or dairy products.

These techniques allow precise and reproducible quantification, compliant with international standards (ISO, EU, USP, AOAC) and adapted to current regulatory requirements.

Why measure vitamin B2?

The dosage of riboflavin is part of an essential quality and regulatory approach:

• Validation of vitamin enrichment in foodstuffs or supplements
• Justification of nutritional claims in accordance with Regulation (EU) No 1924/2006
• Monitoring the stability of vitamin B2 in formulations or during shelf life
• Control of intake for populations with specific needs (vegetarians, elderly people, athletes)
• Verification of the conformity of raw materials (yeasts, plant extracts, nutritional premixes)

Analyzed matrices

Vitamin B2 can be dosed in a wide variety of solid or liquid matrices:

• Food supplements (tablets, powders, capsules, syrups)
• Plant-based drinks or fortified dairy products
• Breakfast cereals or nutrition bars
• Plant extracts (plants, yeasts, algae)
• Animal feed, nutritional premixes
• Enriched cosmetic products (lotions, energizing treatments)

Depending on the matrix, pre-treatment (extraction, purification, derivatization) is performed to reliably release and quantify riboflavin.

Areas of application

Riboflavin dosage is essential for the following industries:

• Food industry : formulation of enriched or functional products
• Nutraceuticals : development of supplements for vitality, skin or vision
• Animal nutrition : formulation of balanced supplements and foods
• Cosmetics : validation of revitalizing treatments containing B2
• Research & development : studies of bioavailability or stability of vitamins

Accredited laboratories for vitamin analysis

YesWeLab relies on a network of accredited laboratories (ISO 17025, COFRAC, etc.) specializing in nutritional analysis and the physical chemistry of vitamins. Our partners have the expertise, equipment, and validated methods necessary to carry out quality analyses that comply with international standards.

We direct you to the most relevant laboratory according to your specifications: matrix, detection threshold, timeframe, applicable standard or specific regulatory need.

Simplified management with YesWeLab

Our digital platform allows you to:

• Centralize your analysis requests
• Track the delivery and processing of your samples
• Access your results online
• Obtain tailor-made scientific support

Since 2020, YesWeLab has been supporting nutrition, health and plant extract manufacturers in the management of their analyses, with a high level of rigor, responsiveness and transparency.

Further analyses recommended

• Vitamin B1 dosage
• Vitamin B6 dosage
• Vitamin B12 dosage
• Nutritional profile

Request your quote today

Add this analysis to your quote via our platform or contact our scientific team. YesWeLab supports you at every stage of your analytical project, from product development to regulatory validation, with ease.