Vitamin analysis

Vitamin analysis allows for the precise quantification of essential micronutrient content in a wide variety of food and nutraceutical matrices. This service is indispensable for ensuring regulatory compliance, validating nutritional claims, optimizing the formulation of fortified products, and guaranteeing consistent quality throughout the production chain. Because vitamins are sensitive to light, oxidation, and heat, their measurement is a key element of quality control in the human food, animal nutrition, plant, and dietary supplement sectors.

Importance of analysis

Controlling vitamin levels is essential for many manufacturers. In the food industry, it allows them to verify the actual contribution of an enriched product, ensure the consistency of nutritional labeling, and monitor the stability of vitamins during storage or the manufacturing process. In nutraceuticals, dosage guarantees the conformity of capsules, tablets, powders, or solutions, and confirms the actual presence of the claimed vitamins. In animal nutrition, it ensures the quality of premixes, complete feeds, and supplements, where vitamin balance plays a key role in animal health, growth, and performance.

Analytical methods

The methods used depend on the type of vitamin, its solubility, and the nature of the matrix.

• For water-soluble vitamins (B1, B2, B3, B6, B9, B12, C), the most common techniques include HPLC, spectrophotometry and LC-MS/MS.
• For fat-soluble vitamins (A, D, E, K), HPLC and LC-MS offer high sensitivity and reliable quantification, even at low concentrations.

Some matrices require specific extraction (enzymatic, alkaline, organic), followed by purification to eliminate interferences. UHPLC methods accelerate analysis while improving the resolution of chromatographic peaks.

Analyzed matrices

Vitamin analysis applies to a wide range of products: dietary supplements in all their forms, fortified beverages, dairy products, infant formula, cereals, plant-based products, fortified oils, agricultural raw materials, processed foods, animal feed, and vitamin premixes. Sample preparation is tailored to each type of matrix to ensure optimal vitamin extraction and reliable quantification.

Interest for professionals

For R&D departments, analysis allows for fine-tuning a formulation to achieve optimal long-term stability. For quality departments, it is essential for documenting regulatory compliance, verifying nutritional labeling, anticipating storage losses, and ensuring the successful launch of new products. Manufacturers can also compare different batches of raw materials, validate natural plant-based sources rich in vitamins, or certify the quality of their fortified products for French and international markets.

YesWeLab Expertise

YesWeLab relies on a network of expert laboratories specializing in nutritional and physicochemical analysis, most of which are ISO 17025 or COFRAC certified and accredited. We select the most suitable method for you (HPLC, LC-MS/MS, spectrophotometry), based on the matrix, the targeted vitamins, and your objectives: quality control, regulatory compliance, formulation optimization, or claim validation. Thanks to our digital platform, you benefit from comprehensive support, simplified tracking, and optimal responsiveness for all your vitamin analyses.

Other relevant analyses from the YesWeLab catalogue

• Polyphenol assay (HPLC, LC-MS)
• Fatty acid profile (GC-FID, GC-MS)
• Mineral analysis
• Antioxidant quantification (HPLC, GC, spectrophotometry)
• Dosage of essential amino acids