Tyrosol (phenol) dosage

Tyrosol analysis allows for the quantification of this natural phenol, which is widely present in olive oil, grape extracts, and certain medicinal plants.
Recognized for its antioxidant and anti-inflammatory properties , tyrosol plays a key role in preventing oxidative stress and protecting cardiovascular health , particularly within the context of the Mediterranean diet.

The analysis is performed by high-performance liquid chromatography (HPLC) , often coupled with mass spectrometry (LC-MS) for sensitive and specific detection. This method allows for obtaining an accurate analytical profile, even in complex matrices such as vegetable oils.

Analytical methods and protocols

The assay is based on a selective extraction of polyphenols, followed by chromatographic separation on a C18 column.

• In HPLC-DAD , detection is performed at approximately 280 nm , a wavelength specific to phenolic compounds.
• In LC-MS/MS , the quantification is finer and allows tyrosol to be distinguished from other related compounds such as hydroxytyrosol or phenolic acids.

The parameters analyzed include:

• The tyrosol concentration (mg/kg or mg/g),
• The overall polyphenolic profile ,
• The purity of the sample ,
• And the stability of the compounds during storage.

IOC/T.20/Doc.29 standards pharmacopoeial methods for plant extracts.

Industrial and scientific applications

Tyrosol dosage is essential in the following sectors:

• Nutraceuticals : quality control of olive or vine extracts, formulation of food supplements with antioxidant properties.
• Agri-food : assessment of the nutritional quality of virgin oils and Mediterranean products.
• Natural cosmetics : promoting tyrosol for its protective and soothing properties on the skin.
• Plant health research : identification of bioactive compounds in plant extracts rich in polyphenols.

This analysis ensures the standardization of raw materials , the traceability of batches , and the enhancement of functional claims ("rich in antioxidants", "made from quality olive oil").

Matrices involved

• Vegetable oils (olive, grapeseed, argan),
• Plant extracts rich in polyphenols,
• Herbal dietary supplements,
• Nutraceutical products with cardioprotective properties
• Cosmetics based on olive extracts.

Further analyses recommended

For a complete characterization of phenolic antioxidants, YesWeLab recommends combining this analysis with:

• Hydroxytyrosol assay for a global evaluation of phenolic compounds in olive oil,
• Vanillin assay for the analysis of aromatic aldehydes,
• Verbascoside assay for monitoring phenylpropanoids,
• Luteolin assay for the complementary study of flavonoids,

YesWeLab's expertise

YesWeLab provides you with its network of partner laboratories accredited to ISO 17025 and COFRAC , specializing in the chromatographic quantification of natural antioxidants .

Thanks to the YesWeLab digital platform , you can:

• Order your analyses online,
• Track your samples and results in real time
• Compare your production batches and centralize your analytical reports.