Tryptophol dosage

The tryptophol assay aims to quantify this metabolite of tryptophan , an indole alcohol naturally present in certain plants, yeasts and fermented products (such as wine, beer or kefir).
A bioactive molecule of growing interest, tryptophol is studied for its sedative, antioxidant and neuroactive properties , as well as for its possible role in microbial cell communication and fermentation processes .

The analysis is based on high-resolution chromatographic separation ( HPLC or LC-MS/MS ), allowing precise identification and quantification even at low concentrations.

Analytical methods and protocols

The quantification of tryptophol is carried out using validated chromatographic methods, adapted to the matrix studied (fermented liquids, plant extracts, nutraceutical powders, etc.).

• HPLC-DAD : detection of tryptophol in the UV, often around 280 nm.
• LC-MS/MS : specific identification of tryptophol and its derivatives (such as 5-hydroxytryptophol), with a very low detection limit.

The parameters measured include:

• The tryptophol concentration (mg/kg or µg/mL),
• The metabolic profile (tryptophan, indole-3-acetaldehyde, indole-3-acetic acid),
• The stability of the compound under storage conditions.

The methods are often based on internal protocols inspired by scientific literature pharmacopoeial references for plant extracts.

Industrial and scientific applications

Tryptophol dosage is used in many areas:

• Nutraceuticals : standardization of plant extracts rich in tryptophan derivatives, formulation of supplements promoting sleep or relaxation.
• Food industry and oenology : fermentation control and monitoring of aromatic compounds in fermented drinks (wine, beer, kombucha).
• Biochemical research : study of tryptophan metabolism and associated indole pathways.
• Cosmetics : exploration of its antioxidant and soothing properties in natural formulas.

This analysis makes it possible to characterize the purity and biochemical quality of the extracts , while guaranteeing their analytical traceability .

Affected matrices

• Plant extracts rich in indole derivatives,
• Fermented drinks (wines, beers, kefirs, kombucha),
• Food supplements or nutraceuticals based on tryptophan,
• Biological or fermentation samples for R&D purposes.

Further analyses recommended

For an in-depth study of the metabolism of tryptophan and its derivatives, YesWeLab recommends combining this analysis with:

• Dosage of tryptophan , amino acid precursor of tryptophol,
• Dosage of tryptamine , a structurally similar bioactive molecule,
• Serotonin dosage , a transformation product of tryptophan in the serotonergic metabolic pathway,
• Melatonin assay to assess final conversion in the sleep pathway.

YesWeLab expertise

YesWeLab relies on a network of ISO 17025 and COFRAC accredited laboratories , experts in chromatographic analyses of natural bioactive compounds .
Our teams support nutraceutical, research and food industry manufacturers in the molecular characterization and analytical validation of their products.

Thanks to the YesWeLab digital platform , you can:

• Order and track your analyses online,
• Access your centralized analytical reports,
• Compare your production batches to ensure consistency in your formulas.