Trigonelline dosage

Trigonelline is an alkaloid derived from niacin (vitamin B3), naturally occurring in several plants, including coffee, fenugreek, alfalfa, and other nutritionally valuable species. Known for its antioxidant, neuroprotective, and hypoglycemic properties, trigonelline is increasingly used as an active ingredient in dietary supplements for metabolic and cognitive management.

The dosage of trigonelline is an essential parameter for:

• Validate the active ingredient content in plant extracts.
• Ensure the standardization of nutraceutical products.
• Check the traceability and quality of plant raw materials.
• Meet the requirements of quality controls and regulations on functional claims.

Analytical methods available: HPLC-DAD and LC-MS/MS

YesWeLab offers trigonelline testing using two laboratory-validated analytical approaches:

• High-performance liquid chromatography (HPLC) with UV detection (DAD), a robust method, suitable for concentrated or standardized extracts.
• Liquid chromatography-coupled mass spectrometry (LC-MS/MS), which allows precise quantification even at very low concentrations, particularly in complex or fermented matrices.
• The choice of method will depend on the type of sample, the expected level of sensitivity and the regulatory framework.

Analyzed matrices

The services offered cover a wide range of products containing trigonelline:

• Plant extracts rich in alkaloids (fenugreek, alfalfa, green coffee, etc.)
• Roasted or unroasted coffee beans
• Functional herbal food supplements
• Vegetable powders or galenic forms (capsules, tablets)

The analysis can also be integrated into a multi-marker study with other compounds of interest, such as caffeine, niacin, chlorogenin, or flavonoids. For a combined approach, explore our caffeine or total flavonoid .

An analytical service adapted to industrial requirements

At YesWeLab, we understand that every analytics project is unique. That's why:

• We select the most relevant laboratory according to your matrices, your regulatory constraints and your volumes.
• We work exclusively with ISO 17025 and COFRAC certified and/or accredited laboratories, guaranteeing reliability and compliance.
• Our scientific team will assist you in choosing the most suitable method (HPLC or LC-MS), interpreting the results and managing your samples.

Industrial application of trigonelline dosage

Trigonelline analysis is useful in many contexts:

• Nutraceutical: formulation of products for memory, concentration, prevention of cellular aging.
• Coffee industry: quality control of beans, certification of green coffee extracts.
• Medicinal plants: traceability of standardized extracts from fenugreek or alfalfa.
• Nutritional supplements: validation of the composition declared on the label.

You can also couple this analysis with the niacin dosage for complete traceability of alkaloids derived from vitamin B3.

Easily order your analysis with YesWeLab

Since 2020, YesWeLab has been supporting manufacturers, brands, and formulators in managing their analytical needs. Thanks to our digital platform, you can:

• Request a quote online in just a few clicks
• Track the status of your samples
• Access your analysis results centrally

Our mission: to save you time, make your data more reliable and simplify access to the most technical analyses on the market.

Need technical advice or an immediate quote?

Contact our scientific team for personalized guidance, to choose the right method, or to check the compatibility of your matrices.