Trigonelline dosage

Trigonelline is an alkaloid derived from niacin (vitamin B3), naturally present in several plants, including coffee, fenugreek, alfalfa, and other nutritionally valuable species. Known for its antioxidant, neuroprotective, and hypoglycemic properties, trigonelline is increasingly used as an active ingredient in dietary supplements designed to support metabolic and cognitive function.

Trigonelline levels are an essential parameter for:

• Validate the active ingredient content in plant extracts.
• To ensure the standardization of nutraceutical products.
• Check the traceability and quality of the plant-based raw materials.
• Meeting the requirements of quality controls and regulations on functional claims.

Available analytical methods: HPLC-DAD and LC-MS/MS

YesWeLab offers trigonelline assay using two laboratory-validated analytical approaches:

• High-performance liquid chromatography (HPLC) with UV detection (DAD), a robust method, suitable for concentrated or standardized extracts.
• Liquid chromatography-mass spectrometry (LC-MS/MS), which allows precise quantification even at very low concentrations, particularly in complex or fermented matrices.
• The choice of method will depend on the type of sample, the expected level of sensitivity, and the regulatory framework.

Analyzed matrices

The services offered cover a wide range of products containing trigonelline:

• Extracts of plants rich in alkaloids (fenugreek, alfalfa, green coffee…)
• Roasted or unroasted coffee beans
• Dietary supplements based on functional plants
• Plant powders or pharmaceutical forms (capsules, tablets)

The analysis can also be integrated into a multi-marker study with other compounds of interest, such as caffeine, niacin, chlorogenin, or flavonoids. For a combined approach, explore our caffeine or total flavonoid .

An analytical service tailored to industrial requirements

At YesWeLab, we know that every analytics project is unique. That's why:

• We select the most relevant laboratory based on your matrices, regulatory constraints and volumes.
• We work exclusively with laboratories certified and/or accredited by ISO 17025, COFRAC, guaranteeing reliability and compliance.
• Our scientific team will assist you in choosing the most suitable method (HPLC or LC-MS), interpreting the results and managing your samples.

Industrial application of trigonelline assay

Trigonelline analysis is useful in many contexts:

• Nutraceuticals: formulation of products for memory, concentration, prevention of cellular aging.
• Coffee industry: quality control of beans, certification of green coffee extracts.
• Medicinal plants: traceability of standardized extracts from fenugreek or alfalfa.
• Nutritional supplements: validation of the composition declared on the label.

You can also combine this analysis with niacin testing for complete traceability of vitamin B3 derived alkaloids.

Order your analysis easily with YesWeLab

Since 2020, YesWeLab has been supporting manufacturers, brands, and formulators in managing their analytical needs. Through our digital platform, you can:

• Request a quote online in just a few clicks
• Track the status of your samples
• Access your analysis results in one central location

Our mission: to save you time, ensure the reliability of your data and simplify access to the most technical analyses on the market.

Need technical advice or an immediate quote?

Contact our scientific team for personalized guidance, to choose the right method or to check the compatibility of your matrices.