Fatty acid analysis (total, profile)

Fatty acid analysis is essential for characterizing the lipid composition of a given matrix. This analysis not only determines the total fatty acid content but also provides a detailed profile ranging from short (C6) to long (C24) chains. Performed by gas chromatography with flame ionization detection (GC-FID), following a lipid transesterification step, this method offers high precision in the quantification and identification of individual fatty acids.

Importance of fatty acid dosage

Fatty acids play a key role in the nutritional, technological, and sensory quality of many food products and plant extracts. Their analysis is essential for:

• the regulatory nutritional labeling of food products,
• the characterization of lipid ingredients in nutraceutical formulations,
• monitoring the quality of raw materials of plant, animal or dairy origin,
• monitoring stability and oxidation during storage,
• differentiating botanical origins or detecting falsifications.

This expertise is aimed at both food manufacturers and those involved in animal nutrition, food supplements or functional cosmetics.

Analytical techniques used

The analysis relies primarily on gas chromatography coupled with flame ionization detection (GC-FID) . This technique, after transesterification of fatty acids to methyl esters, allows for precise separation and quantification. In some cases, GC-MS can also be used for complementary structural identification, particularly when complex profiles or challenging matrices are involved.

Matrices analyzed

Fatty acid analysis is applicable to a wide variety of matrices, including:

• vegetable oils and butters,
• dairy products (cream, cheeses, infant formula),
• plant or algal extracts,
• meat or fish products,
• dietary supplements based on concentrated oils,
• cosmetic formulations containing lipids.

This versatility makes it an essential tool for assessing quality and regulatory compliance in many industrial sectors.

Sector-specific applications

In human food , this analysis provides a detailed nutritional profile, essential for labeling and supporting health claims.
In animal nutrition , it is used to optimize formulations, monitor digestibility, and ensure a lipid balance adapted to physiological needs.
In the nutraceutical , fatty acid analysis is crucial for validating the claimed concentration in fish, flaxseed, or chia oil capsules.
Finally, in cosmetics , it allows for monitoring the fatty acid composition of natural ingredients used for their emollient and moisturizing properties.

Link to other YesWeLab analyses

For a complete assessment, fatty acid analysis can be combined with other analyses available in our catalogue:

• Omega-3 fatty acid testing to specifically target ALA, EPA and DHA,
• Omega-6 fatty acid analysis to characterize the omega-3/omega-6 ratio,
• Peroxide value or TBARS to assess lipid oxidation,
• Acid index to measure the release of free fatty acids.

These additional analyses provide a comprehensive view of the quality and stability of lipid matrices.

YesWeLab Commitment

At YesWeLab, we work with a network of rigorously selected partner laboratories, most of which are certified or accredited (ISO 17025, COFRAC). Each request is studied to adapt the methods to the specific needs of your project, whether it involves food, nutraceutical, or cosmetic products.

Since 2020, our digital platform has simplified analysis management by centralizing requests, logistics tracking, and results delivery. Numerous manufacturers, distributors, and engineering firms already trust us to manage their analyses.

To discuss your needs or obtain a personalized quote, contact our scientific team now.