Total cholesterol measurement

Total cholesterol is the sum of all forms of cholesterol present in a sample, including free and esterified cholesterol. This overall parameter is used as a major indicator of lipid metabolism and nutritional status.

In animal health, its measurement allows for the evaluation of the impact of diets, nutritional supplements, or functional ingredients on lipid balance. It is also essential for the characterization of dietary formulations and for studies in veterinary nutrition.

Principle of analysis

The measurement of total cholesterol relies on several analytical approaches:

• Colorimetric enzymatic methods : simple and fast, suitable for routine analyses.
• ELISA : enabling targeted and reproducible quantification.
• HPLC or GC after saponification : offering precise separation and reliable quantification of the different lipid fractions.

These methods ensure compliance with scientific and regulatory standards, particularly for nutritional labeling.

Applications in animal health

Total cholesterol analysis is used for:

• Evaluate the impact of diets and functional ingredients on lipid metabolism.
• Monitor the effectiveness of nutraceuticals aimed at regulating cholesterol.
• Characterize enriched products or formulations intended for animal nutrition.
• Conduct veterinary clinical studies on the overall lipid profile.

Affected matrices

• Animal serum and plasma
• Lipid extracts
• Dietary products and nutritional formulations
• Animal food supplements

Quality and compliance issues

Measuring total cholesterol allows us to:

• Obtain a complete view of the lipid profile.
• Compare the effect of different nutritional strategies.
• To meet the research needs in veterinary nutrition.
• Ensure compliance with labeling and quality standards.

YesWeLab support

YesWeLab collaborates with a network of accredited partner laboratories (ISO 17025, COFRAC), selected according to your matrices and analytical objectives.

Our digital platform offers you:

• A simple and quick online sample submission
• Real-time monitoring of your analyses
• Reliable, validated and usable results
• The support of our scientific experts for choosing the appropriate methods

Related analyses

• HDL (High-Density Lipoprotein) cholesterol measurement
• LDL (Low-Density Lipoprotein) cholesterol measurement
• Triglyceride levels