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Total cholesterol measurement

Total cholesterol measurement

Technique(s)
Categories: , ,
Sector of activity
Expertise

Physicochemical, Nutritional

Description

Total cholesterol is the sum of all forms of cholesterol present in a sample, including free and esterified cholesterol. This overall parameter is used as a major indicator of lipid metabolism and nutritional status.

In animal health, its dosage allows the assessment of the impact of diets, food supplements or functional ingredients on lipid balance. It is also essential for the characterization of dietary formulations and for veterinary nutrition studies.

Principle of analysis

Total cholesterol measurement is based on several analytical approaches:

  • Colorimetric enzymatic methods : simple and rapid, suitable for routine analyses.
  • ELISA : allowing targeted and reproducible quantification.
  • HPLC or GC after saponification : providing precise separation and reliable quantification of the different lipid fractions.

These methods ensure compliance with scientific and regulatory standards, particularly for nutritional labeling.

Animal health applications

Total cholesterol analysis is used to:

  • To evaluate the impact of dietary patterns and functional ingredients on lipid metabolism.
  • Monitor the effectiveness of nutraceuticals aimed at regulating cholesterol.
  • Characterize enriched products or formulations intended for animal nutrition.
  • Conduct veterinary clinical studies on the overall lipid profile.

Affected matrices

  • Animal serum and plasma
  • Lipid extracts
  • Dietary products and nutritional formulations
  • Food supplements for animals

Quality and compliance issues

Total cholesterol measurement allows:

  • Get a complete view of the lipid profile.
  • Compare the effect of different nutritional strategies.
  • Meeting the needs of veterinary nutrition research.
  • Ensure compliance with labeling and quality standards.

YesWeLab support

YesWeLab collaborates with a network of accredited partner laboratories (ISO 17025, COFRAC), selected according to your matrices and your analytical objectives.

Our digital platform offers you:

  • Quick and easy online sample submission
  • Real-time monitoring of your analyses
  • Reliable, validated and usable results
  • Support from our scientific experts in choosing the right methods

Related analyses

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