Tocopheryl acetate dosage

Tocopheryl acetate, or tocopherol acetate , is an esterified and stabilized form of vitamin E. Used for its antioxidant , it protects formulations against oxidation and contributes to the preservation of the nutritional and cosmetic quality of products. The dosage of tocopheryl acetate is carried out by HPLC-DAD or LC-MS , guaranteeing precise quantification of this molecule in oils, capsules, creams or enriched formulations.

Role and interest of tocopheryl acetate

Tocopheryl acetate is one of the most stable forms of vitamin E, frequently added to products:

• Food : to enrich oils, margarines or nutritional supplements with natural antioxidants.
• Cosmetics : to protect the skin from oxidative stress and improve the preservation of formulations.
• Nutraceuticals : to ensure the stability of capsules or tablets containing fat-soluble vitamins.

Due to its resistance to heat and light , it is preferred in formulations requiring a long shelf life, while converting into active tocopherol in the body after enzymatic hydrolysis.

Objectives and usefulness of tocopheryl acetate dosage

The dosage of this molecule aims to:

• Control the content of active vitamin E in food or nutraceutical products;
• Check the conformity of nutritional or cosmetic claims (e.g. “rich in vitamin E”);
• Ensure the stability of formulations containing compounds sensitive to oxidation;
• Ensure regulatory compliance with technical specifications and composition standards.

Affected matrices include:

• Food supplements (oils, capsules, soft capsules);
• Cosmetic products (creams, serums, enriched vegetable oils);
• Enriched or functional food products;
• Plant extracts for nutritional or pharmaceutical use.

Analytical methods used

The analysis of tocopheryl acetate is based on two complementary approaches:

• HPLC-DAD (high performance liquid chromatography with UV-DAD detection) : reference method for the separation and quantification of different forms of tocopherols and tocopheryl esters.
• LC-MS (liquid chromatography coupled with mass spectrometry) : more specific and sensitive method, used for complex matrices or very low contents.

These techniques distinguish tocopheryl acetate from free tocopherol and other vitamin derivatives. They can be combined with tocopherol (vitamin E) testing to obtain a complete vitamin E profile.

Industrial applications

The dosage of tocopheryl acetate is essential in several sectors:

• Food industry : control of the oxidative stability of enriched oils and fats.
• Cosmetics : validation of the antioxidant content in anti-aging creams and treatments.
• Nutraceuticals : standardization of capsules and supplements rich in vitamin E.
• Pharmaceutical : formulation and quality control of antioxidant excipients.

The analysis is often integrated into an overall check of the vitamin profile, which may include the dosage of retinol (vitamin A) or the dosage of ascorbic acid (vitamin C) .

Carrying out analyses at YesWeLab

Analyses are carried out in ISO 17025 accredited partner laboratories specializing in analytical chemistry and quality control. HPLC-DAD and LC-MS methods are chosen according to the matrix and the desired level of precision.

Each analysis report includes:

• the measured concentration of tocopheryl acetate (mg/kg or %),
• the analytical method used,
• the limit of detection and quantification,
• an interpretation in relation to technical and regulatory specifications.

Since 2020, YesWeLab has been supporting manufacturers in the food, cosmetics and nutraceutical sectors in their vitamin and antioxidant analyses , guaranteeing reliable, rapid results that comply with quality requirements.