Thymoquinone dosage

Thymoquinone is the main bioactive compound extracted from Nigella sativa oil, commonly known as black cumin. This lipophilic quinone molecule is widely recognized for its antioxidant, anti-inflammatory, and immunomodulatory properties. It is attracting increasing interest in the fields of herbal medicine, nutraceuticals, and dietary supplements.

In this context, thymoquinone dosage becomes an essential analytical tool to guarantee the quality, efficacy and conformity of black cumin products.

Why analyze thymoquinone?

Quantitative analysis of thymoquinone allows:

• Check the active ingredient content in Nigella sativa oils or extracts
• Standardize formulations for nutraceutical use
• Ensure compliance with product claims (antioxidant, immune support, etc.)
• Ensure rigorous traceability of plant raw materials

This analysis is particularly recommended during product development, quality control or when changing suppliers.

Analytical methods used

The dosage of thymoquinone is carried out by:

• HPLC-DAD (high performance liquid chromatography with UV detection): robust method for standardized extracts
• LC-MS (liquid chromatography coupled with mass spectrometry): recommended for complex matrices, unrefined oils or mixed products

Our partner laboratories can adapt the method according to the matrix and the expected quantification thresholds.

Affected matrices

The dosage of thymoquinone applies to the following matrices:

• Vegetable oils (cold-pressed black cumin oil, refined or crude)
• Standardized extracts of Nigella sativa (liquid or dry)
• Food supplements (capsules, softgels, powders)
• Fortified food products (functional drinks, nutritional bars)

Compliance, quality and traceability

Thymoquinone is not only a marker of biological activity; it is also an indicator of the quality of black seed products. Accurate dosage allows:

• To detect counterfeits or dilutions of black cumin oil
• To ensure the stability of formulations over time
• To meet the expectations of distributors or retailers in terms of technical data sheets and nutritional values

Further analyses recommended

For a complete characterization of your Nigella sativa extracts or formulations, we recommend:

• Fatty acid dosage 
• Peroxide dosage and acidity index
• Analysis of phenolic composition
• Total flavonoids assay

YesWeLab support

Since 2020, YesWeLab has been supporting manufacturers, formulators, distributors, and laboratories in the management and outsourcing of analyses. Our scientific team identifies the partner laboratory best suited to your needs:

• Validated and sensitive techniques (HPLC, LC-MS)
• ISO 17025 certified and COFRAC accredited laboratories
• Optimized deadlines, negotiated rates, quick quotes

You can manage all of your analysis requests from our secure digital platform and track your results centrally.

Need a quote or a personalized analysis?

Whether you are a black seed oil manufacturer, supplement formulator, or importer, our team is at your disposal.

Click “Add to Quote” to submit your request or contact us for a personalized analysis.