Thymoquinone dosage

Thymoquinone is the main bioactive compound extracted from the oil of Nigella sativa, commonly known as black cumin. This lipophilic quinone molecule is widely recognized for its antioxidant, anti-inflammatory, and immunomodulatory properties. It is attracting increasing interest in the fields of herbal medicine, nutraceuticals, and dietary supplements.

In this context, the measurement of thymoquinone becomes an indispensable analytical tool to guarantee the quality, efficacy and conformity of black cumin-based products.

Why analyze thymoquinone?

Quantitative analysis of thymoquinone allows us to:

• Check the active ingredient content in Nigella sativa oils or extracts
• Standardize nutraceutical formulations
• Ensure compliance with product claims (antioxidant, immune support, etc.)
• Ensuring rigorous traceability of plant-based raw materials

This analysis is particularly recommended during product development, quality control, or when changing suppliers.

Analytical methods used

Thymoquinone levels are measured by:

• HPLC-DAD (high-performance liquid chromatography with UV detection): a robust method for standardized extracts
• LC-MS (liquid chromatography coupled with mass spectrometry): recommended for complex matrices, unrefined oils or mixed products

Our partner laboratories can adapt the method according to the matrix and the expected quantification thresholds.

Matrices involved

The thymoquinone assay applies to the following matrices:

• Vegetable oils (cold-pressed black cumin oil, refined or raw)
• Standardized extracts of Nigella sativa (liquid or dry)
• Dietary supplements (capsules, softgels, powders)
• Fortified food products (functional drinks, nutritional bars)

Compliance, quality and traceability

Thymoquinone is not only a marker of biological activity; it is also an indicator of the quality of black cumin-based products. Precise measurement allows for:

• To detect counterfeit or diluted black cumin oil
• To ensure the stability of the formulations over time
• To meet the expectations of distributors or retailers in terms of technical specifications and nutritional values

Further analyses recommended

For a complete characterization of your extracts or formulations based on Nigella sativa, we recommend:

• Fatty acid dosage 
• Peroxide dosage and acidity index
• Analysis of phenolic composition
• Total flavonoid content assay

YesWeLab support

Since 2020, YesWeLab has been supporting manufacturers, formulators, distributors, and laboratories in managing and outsourcing their analyses. Our scientific team identifies the partner laboratory best suited to your needs:

• Validated and sensitive techniques (HPLC, LC-MS)
• ISO 17025 certified laboratories and COFRAC accredited
• Optimized lead times, negotiated rates, quick quotes

You can manage all your analysis requests from our secure digital platform, and track your results centrally.

Need a quote or a personalized analysis?

Whether you are a black seed oil manufacturer, supplement formulator or importer, our team is at your service.

Click on "Add to quote" to submit your request or contact us for a customized analysis.