Thujone dosage

Thujone analysis is an essential step for manufacturers and researchers working with aromatic plants and their derivatives. These compounds, belonging to the monoterpene family, are naturally present in certain plant species such as mugwort, sage, thuja, and tansy. While they offer interesting sensory and pharmacological properties, thujones also exhibit neurotoxicity when consumed in high doses. Therefore, their quantification is subject to strict regulations, particularly in the alcoholic beverage sector, such as absinthe, but also in essential oils and dietary supplements.

Understanding thujones and their implications

Thujones comprise two main isomers: α-thujone and β-thujone. These lipophilic molecules interact with the central nervous system and are known for their neurotoxic effects when present in excess. Historically associated with absinthe, they are now monitored in numerous plant-based and nutraceutical products.

Their presence is sought for both toxicological and regulatory reasons, but also to guarantee the quality and traceability of the plant extracts used in formulations. Precise measurement of these levels ensures the conformity of finished products, while controlling the health risks associated with their consumption.

Analytical methods for the determination of thujones

Thujone analysis relies primarily on gas chromatography techniques. Two methods are commonly used:

• GC-FID (gas chromatography with flame ionization detection), allowing reliable and reproducible quantification of monoterpenes.
• GC-MS (gas chromatography coupled with mass spectrometry), offering precise identification of isomers and increased sensitivity, suitable for complex matrices.

These analytical approaches guarantee a clear separation of volatile compounds and precise measurement of concentrations, even at low levels. They are performed under standardized conditions to meet regulatory requirements and ensure comparability of results between laboratories.

Sectors affected by thujone analysis

Thujone analysis is particularly relevant in several industrial sectors:

• Nutraceutical industry: control of plant extracts used in food supplements to verify their safety and regulatory compliance.
• Aromatic and medicinal plants: qualitative and quantitative monitoring of essential oils, hydroalcoholic extracts and macerates.
• Alcoholic beverages: control of thujone levels in absinthe, liqueurs or other spirits derived from wormwood or sage.
• Pharmaceutical industry and herbalism: mastery of active ingredients for the development of therapeutic or traditional products.

This diversity of applications demonstrates the importance of analytical expertise to secure formulations and ensure the conformity of marketed products.

Typically analyzed matrices

Thujones can be quantified in a wide variety of matrices:

• Essential oils derived from sage, thuja or mugwort
• Plant extracts intended for use in food supplements
• Alcoholic drinks such as absinthe or aromatic liqueurs
• Plant-based pharmaceutical and cosmetic products

Each matrix presents specific analytical challenges, requiring technical expertise and adapted methods to obtain reliable results.

A strict regulatory framework

European regulations set precise thresholds for thujone levels in food and beverages. For example, Directive 2008/84/EC establishes maximum limits for thujone in alcoholic beverages and flavored foods. These standards aim to protect consumers from neurotoxic effects and to harmonize industry practices.

For manufacturers and distributors, complying with these limits is a major compliance issue, determining whether products can be placed on the market. Using specialized laboratories ensures results that are validated and recognized by the authorities.

YesWeLab support for your thujone analyses

At YesWeLab, we offer our expertise and network of partner laboratories to support you in thujone analysis. Our partners are rigorously selected based on their accreditations (ISO 17025, COFRAC) and their mastery of analytical techniques suited to your matrices.

Thanks to our digital platform, you can easily submit your samples, track your analyses, and receive your results securely. Our scientific teams are committed to meeting your specific needs with precision and responsiveness, advising you on the most suitable methods and guaranteeing optimized turnaround times.

Many manufacturers, distributors, and engineering firms already trust us to secure their analytical projects. Contact our team to learn more or to obtain a personalized quote for your thujone analyses.

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