Theobromine dosage

Theobromine is a molecule from the methylxanthine family, structurally similar to caffeine. Naturally present in cocoa beans, tea, guarana, and certain medicinal plants, it is known for its mild stimulant effects, its diuretic properties, and its vasodilatory action.

Theobromine dosage is essential in several industrial contexts:

• In human nutrition, to control the active ingredient content of cocoa or tea extracts used in functional drinks, food supplements or energy products.
• In nutraceuticals, to standardize cocoa or guarana-based extracts integrated into health formulas.
• In animal nutrition, in order to assess the safety of formulations (theobromine being toxic to certain species, notably dogs).
• In the food industry, to certify the composition of cocoa-based products, guarantee regulatory compliance or authenticate a material origin.

Proposed analytical methodologies

Theobromine is analyzed by liquid chromatography:

• HPLC-DAD (High Performance Liquid Chromatography – Diode Array Detection): standard method used for simple or concentrated matrices.
• LC-MS/MS (chromatography coupled with tandem mass spectrometry): recommended for complex matrices, or when very low detection limits are required.

We adapt the method according to your matrices and your objectives:

• Finished products (chocolates, drinks, energy bars)
• Ingredients (cocoa solids, tea powder, guarana)
• Raw or processed plant materials
• Food supplements (capsules, tablets, soluble powders)
• Animal feed

A key dosage for quality, compliance and safety

The dosage of theobromine allows:

• Check compliance with internal or regulatory specifications
• Ensuring consistency of herbal formulations
• Detect cross-contamination (especially in animal feed)
• Assess residual levels as part of quality controls

This analysis can also be cross-referenced with that of other methylxanthines, such as caffeine and theophylline, for a comprehensive view of the bioactive profile of the extracts.

Related analyses to explore

To go further in the characterization of your extracts or supplements, we recommend the following analyses:

• Caffeine Dosage – For Tea, Coffee or Guarana Extracts
• Theanine Dosage – Complementary Relaxing Amino Acid, Extracted from Green Tea
• Glabridin dosage – Licorice flavonoid with antioxidant effects

Entrust your analyses to YesWeLab

Since 2020, YesWeLab has been supporting nutrition, health, and food manufacturers with their analytical needs. Thanks to our digital platform and network of more than 200 partner laboratories, we offer you a reliable, fast, and ISO 17025 / COFRAC-compliant solution.

• Laboratory selection based on matrix, method and your sector
• Analysis in accordance with current standards
• Available and responsive scientific team
• Online Sample Tracking

Need a quote or advice?
Add this analysis to your request and specify your requirements (thresholds, matrices, standards to be met).
Our team will respond within 24 to 48 hours with a tailored proposal.