T2/HT2 toxin assay

T-2 and HT-2 toxins are trichothecene mycotoxins produced primarily by fungi of the genus Fusarium . These natural contaminants develop in cereals and their derivatives during storage or under climatic conditions favorable to mold growth.

Their presence poses a risk to human and animal health immunotoxic, hematotoxic, and digestive effects . Measuring T-2 and HT-2 toxins helps ensure food safety regulatory compliance according to Regulation (EU) No 1881/2006 EFSA recommendations .

Analytical methods: HPLC and LC-MS/MS

The analysis of mycotoxins T-2 and HT-2 relies on high-precision chromatographic techniques:

• HPLC (high-performance liquid chromatography) : used for separation and quantification after extraction and purification on immunoaffin column.
• LC-MS/MS (liquid chromatography coupled with tandem mass spectrometry) : reference method allowing the simultaneous detection of several mycotoxins (T-2, HT-2, DON, ZEA, fumonisins, etc.) at trace levels .

These methods ensure excellent sensitivity (ng/kg) and maximum reliability ISO 17025 accredited laboratories .

Industrial and regulatory applications

The measurement of T-2 and HT-2 toxins is applicable in:

• Human nutrition : control of raw materials (wheat, corn, oats, rice) and finished products (flours, breads, biscuits, cereals).
• Animal nutrition : verification of compound feeds, corn silage, oilseed cakes and agricultural by-products.
• Nutraceuticals and plant extracts : securing powders and plant raw materials used in food supplements.
• Regulatory studies and exports : compliance with European and international thresholds.

This test can be combined with the measurement of zearalenone (ZEA) or deoxynivalenol (DON) for a complete multi-mycotoxin analysis .

Affected matrices

YesWeLab's partner laboratories perform T-2 and HT-2 toxin assays on:

• Cereals and raw grains (wheat, corn, barley, oats, rice)
• Flours, brans, flakes and derivative products
• Compound feed for animals
• Nutraceutical plant extracts and powders

Each protocol is adjusted according to the matrix , the expected level of contamination and the regulatory framework .

YesWeLab expertise

At YesWeLab , we collaborate with a network of ISO 17025 and COFRAC accredited laboratories , specializing in the detection and quantification of mycotoxins .

Our scientific team assists you in choosing the appropriate method , managing the logistics of samples , and monitoring analyses via our digital platform . You benefit from a centralized, fast service that complies with European requirements.

Since 2020, YesWeLab has been supporting agri-food industries, animal feed manufacturers and nutraceutical players in their food safety analyses .

Order a T-2 and HT-2 toxin analysis

Do you want to monitor the presence of T-2 and HT-2 toxins in your cereals, flours or plant products?
Submit your request via our platform or contact our scientific team for personalized support .

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