g-strophanthin assay

γ-Strophanthin, also called ouabain, is a cardiotonic glycoside extracted primarily from Strophanthus gratus . It belongs to the class of cardiotonic glycosides, traditionally used for their effect on myocardial function. Although its therapeutic use is now strictly regulated, it is still analyzed in nutraceutical, ethnobotanical, and regulatory contexts for:

• Securing plant extracts containing active glycosides,
• Avoid any contamination or excessive dosage,
• Standardize plant-based preparations,
• To meet traceability and compliance requirements.

Why measure g-strophantin?

Measuring the concentration of γ-strophanthin is essential for assessing the concentration of a highly active ingredient. Occurring naturally in certain plants, its potential toxicity warrants rigorous analytical control. This analysis allows for:

• To validate the conformity of botanical extracts intended for nutraceutical or cosmetic use,
• To prevent the risk of adverse effects from overdose,
• To guarantee regulatory transparency, particularly in raw material exports.

Analytical methods used

The analysis is performed according to the most reliable analytical standards:

HPLC (High Performance Liquid Chromatography)

High-performance liquid chromatography with UV or DAD detection allows for precise quantification in simple extracts or tinctures.

LC-MS or LC-MS/MS

Liquid chromatography-mass spectrometry (LC-MS/MS) is preferred for complex matrices or low concentrations, ensuring optimal specificity and sensitivity.

Affected matrices

• Standardized plant extracts with tonic or cardiotonic properties,
• Mother tinctures and galenic preparations derived from Strophanthus gratus ,
• Herbal dietary supplements containing glycosides,
• Ethnobotanical products or traditional preparations.

Industrial sectors concerned

• Nutraceuticals and phytotherapy: verification of active ingredient levels in medicinal plants;
• Scientific and clinical research: studies on the physiological effects of g-strophantin;
• Plant extracts industry: quality control and standardization of raw materials;
• Regulatory control and safety: compliance with set thresholds or exclusion of prohibited active substances.

Recommended related analyses (internal links)

To supplement your analysis of cardioactive plant extracts, you can also consult:

• Dosage of colchicine – toxic mitotic-type alkaloid to be monitored in extracts;
• Dosage of galegine – alkaloid with hypoglycemic effect from Galega officinalis ;
• Total polyphenol assay – monitoring of overall antioxidant activity;
• Dosage of diosmetin – methylated flavone with vasoprotective effect.

YesWeLab's analytical expertise

At YesWeLab, we select specialized partner laboratories, certified ISO 17025 and/or accredited COFRAC, capable of meeting the specific requirements related to the analysis of natural glycosides.

With our digital platform, you benefit from:

• Quick access to quotes,
• Personalized scientific support,
• Traceability of samples and results,
• Validated methods for all types of matrices.

Since 2020, we have been supporting manufacturers, formulators, R&D laboratories and distributors in the secure management of their analyses.

Order your g-strophanthin dosage

Need to measure the g-strophanthin content in your plant extracts or phytotherapeutic products? Our team can guide you through:

• The choice of the appropriate analytical method (HPLC, LC-MS);
• The establishment of a customized dosage protocol;
• Fast and reliable analysis of your samples.