Squalene dosage

allows Squalene analysis for the quantification of this natural lipophilic triterpene found in many vegetable oils (notably olive and amaranth oils) as well as in the liver of certain fish.
Used as an antioxidant, moisturizing agent , and precursor to cholesterol biosynthesis, squalene is a marker of nutritional and cosmetic quality. Its measurement is essential for the standardization of raw materials, the control of oil purity, and the characterization of active ingredients in nutraceutical and cosmetic products.

Analytical methods

Squalene analysis relies on two main chromatographic approaches:

• GC-FID (gas chromatography with flame ionization detection) : reference method for the quantification of squalene in oils and fatty extracts, offering excellent accuracy.
• HPLC-DAD (high-performance liquid chromatography) : used for complex formulations (cosmetics, supplements) or matrices containing other lipid compounds.

Analyses can be performed according to AOCS standards, ISO 12228-2 or internal protocols validated according to ISO 17025, guaranteeing reliable and reproducible results.

Industrial applications

Squalene assay is widely used in several sectors:

• Nutraceutical : standardization of vegetable oils rich in squalene (olive, amaranth, rice bran).
• Cosmetics : control of purity and moisturizing efficacy of products containing squalene or squalane.
• Agri-food : traceability and verification of the quality of edible oils.
• Pharmaceutical : control of lipid formulations or vaccine adjuvants.

Matrices analyzed

• Vegetable oils (olive, amaranth, rice, wheat germ)
• Natural lipid extracts
• Oily dietary supplements
• Cosmetic products (creams, serums, treatment oils)
• Functional ingredients for nutrition or dermocosmetics

Further analyses recommended

For a complete assessment of lipid and antioxidant profiles, YesWeLab recommends:

• Stigmastadiene assay : an indicator of oil refining,
• Cholesterol and phytosterol : characterization of the sterol profile,
• Oxidation stability of fats and oils : measurement of resistance to oxidation,
• Peroxide value : assessment of primary rancidity.

YesWeLab's expertise

YesWeLab relies on a network of partner laboratories accredited to ISO 17025 and COFRAC, specializing in the analysis of lipids, plant extracts, and functional ingredients.
Our digital platform centralizes your analytical needs and facilitates the complete management of tests : from ordering to receiving results.

Need to control the squalene content of your oils or formulations?
Contact our scientific team today for tailored support.