Squalene dosage

Squalene analysis allows for the quantification of this natural lipophilic triterpene found in many vegetable oils (notably olive and amaranth oils ) as well as in the liver of certain fish .
Used as an antioxidant , moisturizing agent , and precursor to cholesterol biosynthesis , squalene is a marker of nutritional and cosmetic quality . Its measurement is essential for the standardization of raw materials , the control of oil purity , and the characterization of active ingredients in nutraceutical and cosmetic products.

Analytical methods

Squalene analysis relies on two main chromatographic approaches:

• GC-FID (gas chromatography with flame ionization detection) : reference method for the quantification of squalene in oils and fatty extracts, offering excellent accuracy.
• HPLC-DAD (high-performance liquid chromatography) : used for complex formulations (cosmetics, supplements) or matrices containing other lipid compounds.

Analyses can be performed according to AOCS standards, ISO 12228-2 or internal protocols validated according to ISO 17025 , guaranteeing reliable and reproducible results.

Industrial applications

Squalene assay is widely used in several sectors:

• Nutraceutical : standardization of vegetable oils rich in squalene (olive, amaranth, rice bran).
• Cosmetics : purity control and moisturizing efficacy of products containing squalene or squalane.
• Agri-food : traceability and verification of the quality of edible oils.
• Pharmaceutical : control of lipid formulations or vaccine adjuvants.

Matrices analyzed

• Vegetable oils (olive, amaranth, rice, wheat germ)
• Natural lipid extracts
• Oily dietary supplements
• Cosmetic products (creams, serums, treatment oils)
• Functional ingredients for nutrition or dermocosmetics

Further analyses recommended

For a complete assessment of lipid and antioxidant profiles, YesWeLab recommends:

• Stigmastadiene assay : an indicator of oil refining.
• Cholesterol and phytosterol : characterization of the sterol profile,
• Oxidation stability of fats and oils : measurement of resistance to oxidation,
• Peroxide value : assessment of primary rancidity.

YesWeLab's expertise

YesWeLab relies on a network of partner laboratories accredited to ISO 17025 and COFRAC , specializing in the analysis of lipids, plant extracts, and functional ingredients .
Our digital platform centralizes your analytical needs and facilitates the complete management of tests : from ordering to receiving results.

Need to control the squalene content of your oils or formulations?
Contact our scientific team today for tailored support.