Squalene dosage

The dosage of squalene makes it possible to quantify this natural lipophilic triterpene present in many vegetable oils (notably olive oil and amaranth oil ) as well as in the liver of certain fish .
Used as an antioxidant , moisturizing agent and precursor of cholesterol biosynthesis , squalene is a marker of nutritional and cosmetic quality . Its dosage is essential for the standardization of raw materials , the control of the purity of oils , and the characterization of active ingredients in nutraceutical and cosmetic products.

Analytical methods

Squalene analysis is based on two main chromatographic approaches:

• GC-FID (gas chromatography with flame ionization detection) : reference method for the quantification of squalene in oils and fatty extracts, offering excellent precision.
• HPLC-DAD (high performance liquid chromatography) : used for complex formulations (cosmetics, supplements) or matrices containing other lipid compounds.

Analyses can be performed according to AOCS, ISO 12228-2 standards or internal protocols validated according to ISO 17025 , guaranteeing reliable and reproducible results.

Industrial applications

Squalene dosage is widely used in several sectors:

• Nutraceutical : standardization of vegetable oils rich in squalene (olive, amaranth, rice bran).
• Cosmetics : control of purity and moisturizing effectiveness of products containing squalene or squalane.
• Food industry : traceability and verification of the quality of edible oils.
• Pharmaceutical : control of lipid formulations or vaccine adjuvants.

Analyzed matrices

• Vegetable oils (olive, amaranth, rice, wheat germ)
• Natural lipid extracts
• Oily food supplements
• Cosmetic products (creams, serums, care oils)
• Functional ingredients for nutrition or dermocosmetics

Further analyses recommended

For a complete assessment of lipid and antioxidant profiles, YesWeLab recommends:

• Stigmastadiene dosage : indicator of oil refining,
• Cholesterol and phytosterol : characterization of the sterol profile,
• Oxidation stability of fats and oils : measurement of resistance to oxidation,
• Peroxide value : evaluation of primary rancidity.

YesWeLab expertise

YesWeLab relies on a network of ISO 17025 and COFRAC accredited partner laboratories , specialized in the analysis of lipids, plant extracts and functional ingredients .
Our digital platform centralizes your analytical needs and facilitates complete test management : from ordering to receiving results.

Need to control the squalene content of your oils or formulations?
Contact our scientific team today for personalized support.