Skin absorption – OECD 428

Dermal absorption testing according to OECD 428 is an in vitro method for assessing the amount of a chemical substance that penetrates human skin. This technique is essential for determining potential systemic exposure to substances present in cosmetic, agrochemical, or industrial products.

The objective is to quantify the transdermal penetration of a substance applied to excised skin, thus providing crucial data for risk assessment and product safety.

Sample Matrix: This analysis is applicable to various matrices, including cosmetic formulations, plant protection products, industrial chemicals, and other substances intended to come into contact with human skin.

The method relies on the use of diffusion cells, where the skin separates a donor chamber containing the test substance from a receptor chamber containing a receiving fluid. After a defined exposure period, the receptor fluid is analyzed to quantify the substance and its possible metabolites, usually using techniques such as liquid or gas chromatography coupled with mass spectrometry.

At YesWeLab, we collaborate with a rigorously selected network of laboratories, most of which are certified and/or accredited (ISO 17025, COFRAC, etc.). These laboratories are chosen based on your specific needs, the matrices to be analyzed, and the required analytical techniques or methods.
If you have any specific requirements, please do not hesitate to specify them when submitting your request: our scientific team will do everything possible to respond accurately and responsively.
Since 2020, many manufacturers, distributors, and design offices have trusted us to manage their analyses, entrusting us with their samples via our digital platform.
To find out more or submit a specific request, contact our team now.