Rutin assay

Rutin, also known as rutinoside or sophorin, is a glycosylated flavonoid naturally present in many medicinal and edible plants. It is found notably in buckwheat, Japanese pagoda tree (Sophora japonica), citrus fruits, apples, and green tea. It is particularly valued for its antioxidant , vasoprotective , and anti-inflammatory , making it a marker of major interest in the fields of nutraceuticals and functional cosmetics.

This compound plays a protective role for the cardiovascular system and helps limit oxidative stress. Its dosage is crucial for standardizing plant extracts, validating the claimed active ingredient content, and ensuring the compliance of marketed formulations.

Importance of rutin measurement

Rutin testing addresses several needs:

• Quality control of plant-based raw materials used in the nutraceutical industry.
• Validation of the efficacy and compliance of a food supplement enriched with flavonoids.
• Evaluation of the stability of cosmetic formulations containing antioxidant extracts.
• Detection of the natural variability of botanical extracts and verification of their authenticity.

Analytical methods

Rutin analysis is carried out mainly by high-performance liquid chromatography (HPLC) coupled with UV-Vis detection, or by mass spectrometry (LC-MS) for finer and more specific quantification, particularly in complex matrices.

• HPLC -UV is used for routine analysis and standardization of extracts.
• LC -MS/MS allows confirmation of the compound's identity and provides sensitive detection even at low concentrations.

These methods guarantee accurate quantification, essential to meet traceability and regulatory compliance requirements.

Affected matrices

Rutin can be measured in a wide variety of matrices:

• Medicinal plants : buckwheat, sophora, citrus fruits, tea.
• Plant extracts : powders, hydroalcoholic solutions, tinctures.
• Dietary supplements : capsules, tablets, liquid formulations.
• Cosmetic products : serums, antioxidant creams, dermocosmetic formulations.

Regulatory issues

In the nutraceutical sector, rutin measurement ensures product compliance with health claims and labeling requirements. In the cosmetics industry, it meets the quality and safety requirements of protective and antioxidant formulations.

Furthermore, the standardization of extracts rich in flavonoids such as rutin is an essential criterion for manufacturers in order to ensure consistent effectiveness from batch to batch.

Related analyses in the YesWeLab catalog

Rutin levels are often measured in conjunction with other analyses targeting flavonoids and polyphenols:

• Quercetin assay (aglycone of rutin, major antioxidant).
• Total flavonoid assay for overall quantification of polyphenols.
• Dosage of chlorogenic acid often present in the same plant extracts.
• Catechin dosage for tea and other antioxidant-rich matrices.

YesWeLab expertise

At YesWeLab, we work with a network of laboratories selected for their expertise and accreditation (ISO 17025, COFRAC). We choose the partner best suited to your needs, according to your matrices and regulatory requirements.

Our digital platform simplifies the management of your analyses: quote requests, sample tracking, and centralized results reception. Since 2020, numerous manufacturers, distributors, and engineering firms have entrusted us with their analyses, benefiting from our scientific support and responsiveness.