Ruscogenin dosage

Ruscogenin is a natural steroidal saponin extracted from butcher's broom ( Ruscus aculeatus ), known for its venotonic and anti-inflammatory properties. It is commonly used in cosmetic formulations and dietary supplements designed to promote venous circulation and reduce the feeling of heavy legs. The dosage of ruscogenin ensures the quality, purity, and conformity of the plant extracts used in these products.

This analysis is performed by high-performance liquid chromatography (HPLC) , a reference method for the separation and quantification of steroidal saponins.

Principle and operation of the method

The analysis is based on the selective extraction of ruscogenin from plant matrices or finished formulations (dry extracts, creams, capsules). The sample is then injected into an HPLC system equipped with a UV or DAD detector.

The separation of the compounds is based on their interactions with the stationary phase of the column, allowing ruscogenin to be isolated from other saponins or plant constituents. This method guarantees reliable and reproducible quantification that conforms to international analytical standards.

Analytical performance and related analyses

HPLC offers excellent sensitivity and specificity, making it suitable for complex products derived from medicinal plants. It also allows for the verification of ruscogenin content in various matrices, whether solid, liquid, or semi-solid.

For a more complete characterization of butcher's broom extracts or other venotonic formulations, this analysis can be combined with:

• the dosage of diosmin ,
• the dosage of hesperidin,
• the dosage of troxerutin,
• or the dosage of total flavonoids.

These additional analyses make it possible to determine the polyphenolic and saponinic profile of the natural extracts and to evaluate their biological potential.

Applications and areas of use

Ruscogenin testing is applicable in several sectors:

• Nutraceuticals : control of the active ingredient content in circulatory or draining food supplements.
• Cosmetics : evaluation of topical formulations intended to improve venous comfort and reduce swelling.
• Research & development : standardization of Ruscus aculeatus and validation of the stability of active ingredients.
• Quality control : verification of batch conformity according to manufacturer specifications and applicable regulatory standards.

This analysis contributes to the reliability of products containing butcher's broom and to highlighting its natural vein-toning effects.

The advantages of the YesWeLab service

YesWeLab collaborates with a network of partner laboratories accredited to ISO 17025 and COFRAC, specializing in the analysis of plant bioactive compounds. Each project is assigned to the most suitable laboratory based on the matrix, method, and required precision.

Thanks to its digital platform, YesWeLab simplifies the management of your analyses: requesting quotes, tracking samples, and receiving results online. Since 2020, numerous nutraceutical and cosmetic companies have trusted us for the standardization and quality control of their natural extracts.

For any specific request regarding ruscogenin assay, contact our scientific team to obtain a personalized quote and support tailored to your needs.