Vitamin B2 (riboflavin) dosage

Riboflavin analysis , more commonly known as vitamin B2 , allows for the quantification of this essential water-soluble vitamin in various food products, supplements, and nutraceutical formulations. Involved in numerous enzymatic reactions, vitamin B2 plays a central role in cellular energy production, the metabolism of amino acids, lipids, and carbohydrates, as well as in maintaining healthy skin and mucous membranes. Its measurement is crucial for manufacturers seeking to guarantee nutritional intake, substantiate a claim, or enrich a plant- or animal-based product matrix.

Analytical methods: HPLC-DAD, LC-MS and fluorescence

YesWeLab offers several reference techniques for riboflavin analysis, depending on the nature of the matrix and the required precision:

• HPLC-DAD (high-performance liquid chromatography with UV-DAD detection): classic and reliable method for food products and standard extracts.
• LC-MS (liquid chromatography coupled with mass spectrometry): particularly suitable for complex matrices or trace assays.
• Fluorescence after derivatization: a highly sensitive method, used especially for beverages or dairy products.

These techniques allow for precise and reproducible quantification, in accordance with international standards (ISO, EU, USP, AOAC) and adapted to current regulatory requirements.

Why measure vitamin B2 levels?

Riboflavin testing is part of an essential quality and regulatory process:

• Validation of vitamin enrichment in foodstuffs or supplements
• Justification of nutritional claims in accordance with Regulation (EU) No 1924/2006
• Monitoring the stability of vitamin B2 in formulations or during shelf life
• Control of intake for populations with specific needs (vegetarians, the elderly, athletes)
• Verification of the conformity of raw materials (yeasts, plant extracts, nutritional premixes)

Analyzed matrices

Vitamin B2 can be measured in a wide variety of solid or liquid matrices:

• Dietary supplements (tablets, powders, capsules, syrups)
• Fortified plant-based drinks or dairy products
• Breakfast cereals or nutrition bars
• Plant extracts (plants, yeasts, algae)
• Animal feed, nutritional premixes
• Enriched cosmetic products (lotions, energizing treatments)

Depending on the matrix, a pretreatment (extraction, purification, derivatization) is carried out to reliably release and quantify riboflavin.

Areas of application

Riboflavin assay is essential for the following industries:

• Food processing : formulation of enriched or functional products
• Nutraceuticals : development of supplements designed for vitality, skin or vision
• Animal nutrition : formulation of supplements and balanced feeds
• Cosmetics : validation of revitalizing skincare products containing B2
• Research & development : studies on the bioavailability or stability of vitamins

Accredited laboratories for vitamin analysis

YesWeLab relies on a network of accredited laboratories (ISO 17025, COFRAC, etc.) specializing in nutritional analysis and the physicochemical properties of vitamins. Our partners possess the expertise, equipment, and validated methods necessary to perform high-quality analyses that comply with international standards.

We direct you to the most relevant laboratory according to your specifications: matrix, detection threshold, time frame, applicable standard or specific regulatory need.

Simplified management with YesWeLab

Our digital platform allows you to:

• Centralize your analysis requests
• Track the delivery and processing of your samples
• Access your results online
• Obtain tailored scientific support

Since 2020, YesWeLab has been supporting nutrition, health and plant extract manufacturers in managing their analyses, with a high level of rigor, responsiveness and transparency.

Further analyses recommended

• Vitamin B1 dosage
• Vitamin B6 dosage
• Vitamin B12 dosage
• Nutritional profile

Request your quote today

Add this analysis to your quote via our platform or contact our scientific team. YesWeLab supports you at every stage of your analytical project, from product development to regulatory validation, with ease.