Resveratrol dosage

Resveratrol is a natural polyphenol belonging stilbene family , known for its antioxidant, anti-inflammatory and cardioprotective properties . Present in grapes , red wine , Japanese knotweed ( Polygonum cuspidatum ) or blueberries , it constitutes a key biomarker in the evaluation of the quality of plant extracts and nutraceutical products.

Resveratrol assay is performed by HPLC-DAD or LC-MS/MS , allowing precise identification and quantification of the compound, whether in trans-resveratrol (active) or cis-resveratrol (secondary isomer) form. This analysis is essential to ensure the purity , stability and standardization of formulations for health, antioxidant or anti-aging purposes.

Resveratrol: a major bioactive polyphenol

Resveratrol (3,5,4′-trihydroxystilbene) is a phenolic compound produced by certain plants in response to environmental stress (infection, UV, drought). It occurs in two isomeric forms:

• trans-resveratrol , biologically active and the most researched;
• cis-resveratrol , less stable and often resulting from the transformation of the first.

Its presence is particularly notable in:

• grape skin and red wine ,
• Japanese knotweed , rich in natural resveratrol used for standardized extracts,
• peanuts , blackberries , blueberries and other colorful fruits .

Resveratrol is often studied for its effects on cellular longevity , cardiovascular health and protection against oxidative stress , hence its growing importance in functional nutrition and natural cosmetics .

Objectives and interest of resveratrol dosage

The dosage of resveratrol by chromatography allows:

• quantify the total resveratrol content in plant extracts, wines or food supplements,
• assess the trans/cis proportion to ensure product stability and efficacy,
• standardize batches of natural ingredients in nutraceutical sectors,
• verify the regulatory compliance of enriched formulations,
• support antioxidant and nutritional claims on product labels.

The most commonly analyzed matrices include:

• plant extracts ( notably Japanese knotweed),
• food supplements in capsules, powders or liquids,
• wines and functional drinks ,
• natural cosmetic products rich in polyphenols.

Analytical methods used

Resveratrol is analyzed by HPLC-DAD or LC-MS/MS depending on the nature and complexity of the matrix:

• HPLC-DAD (liquid chromatography with UV-Visible detection) : reference method for the quantification of resveratrol in plant extracts and finished products. Detection is carried out around 306 nm, the characteristic wavelength of stilbene.
• LC-MS/MS (liquid chromatography coupled with mass spectrometry) : a more sensitive technique allowing specific identification of isomers and ensuring reliable quantification even at low concentrations.

This analysis can be supplemented by additional dosages such as:

• Pterostilbene dosage,
• Dosage of total polyphenols,
• Dosage of total flavonoids,
• Anthocyanin assay,
  to establish a complete antioxidant profile of the sample.

Industrial and regulatory applications

Resveratrol dosage is used in many sectors:

• Nutraceuticals : control of resveratrol content in food supplements and plant extracts for health or anti-aging purposes.
• Food industry and oenology : monitoring of polyphenol levels in wine, grapes or juices, indicator of quality and antioxidant activity.
• Natural cosmetics : formulation of anti-aging and antioxidant active ingredients in skin care.
• Scientific research : studies of bioavailability, antioxidant efficacy and synergistic interactions with other phenolic compounds (quercetin, catechins, pterostilbene).

From a regulatory perspective, extracts titrated in resveratrol must meet purity and traceability requirements, particularly within the framework of European regulations on food ingredients and supplements .

Carrying out analyses at YesWeLab

YesWeLab collaborates with a network of laboratories expert in chromatography of polyphenols and plant metabolites HPLC-DAD , UHPLC and LC-MS/MS instruments ISO 17025 requirements .

Tests are carried out according to validated methods, ensuring accuracy , reproducibility and full traceability of results. Analysis reports include:

• the total resveratrol content (mg/g or mg/L) ,
• the trans/cis distinction ,
• and compliance recommendations for industrial applications.

Since 2020, YesWeLab has been supporting players in the nutraceutical , agri -food and natural cosmetics in the analytical control of their polyphenolic extracts and in the promotion of their active ingredients.

Thanks to its digital platform , YesWeLab centralizes your analytical needs, from the request for a quote to the receipt of results, guaranteeing responsive and personalized scientific support.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors: Nutraceuticals, food, plants, natural cosmetics
• Measured parameters: Resveratrol (trans and cis)
• Normative references: ISO 17025, validated internal methods

For any polyphenol analysis or antioxidant profile development needs, contact our scientific team for a personalized quote.