Resveratrol dosage

Resveratrol is a natural polyphenol belonging to the stilbene , known for its antioxidant, anti-inflammatory, and cardioprotective properties . Found in grapes , red wine , Japanese knotweed ( Polygonum cuspidatum ) , and blueberries , it is a key biomarker in evaluating the quality of plant extracts and nutraceutical products.

Resveratrol is measured using HPLC-DAD or LC-MS/MS , allowing for precise identification and quantification of the compound, whether in its trans-resveratrol (active) or cis-resveratrol (secondary isomer) form. This analysis is essential to guarantee the purity , stability , and standardization of formulations intended for health, antioxidant, or anti-aging purposes.

Resveratrol: a major bioactive polyphenol

Resveratrol (3,5,4′-trihydroxystilbene) is a phenolic compound produced by certain plants in response to environmental stress (infection, UV radiation, drought). It exists in two isomeric forms:

• trans-resveratrol , biologically active and the most sought after;
• cis-resveratrol , less stable and often derived from the transformation of the former.

Its presence is particularly notable in:

• the skin of the grapes and red wine ,
• Japanese knotweed , rich in natural resveratrol used for standardized extracts,
• peanuts , blackberries , blueberries and other colorful fruits .

Resveratrol is often studied for its effects on cellular longevity , cardiovascular health and protection against oxidative stress , hence its growing importance in functional nutrition and natural cosmetics .

Objectives and purpose of resveratrol assay

The measurement of resveratrol by chromatography allows us to:

• quantify the total resveratrol content in plant extracts, wines or food supplements,
• evaluate the trans/cis ratio to ensure product stability and efficacy,
• to standardize batches of natural ingredients in nutraceutical supply chains,
• verify the regulatory compliance of enriched formulations,
• support antioxidant and nutritional claims on product labels.

The most commonly analyzed matrices include:

• plant extracts ( particularly Japanese knotweed),
• dietary supplements in capsule, powder or liquid form,
• wines and beverages ,
• Natural cosmetic products rich in polyphenols.

Analytical methods used

Resveratrol is analyzed by HPLC-DAD or LC-MS/MS depending on the nature and complexity of the matrix:

• HPLC-DAD (UV-Visible Detection Liquid Chromatography) : the reference method for quantifying resveratrol in plant extracts and finished products. Detection occurs around 306 nm, the characteristic wavelength of stilbene.
• LC-MS/MS (liquid chromatography coupled with mass spectrometry) : a more sensitive technique allowing specific identification of isomers and ensuring reliable quantification even at low concentrations.

This analysis can be supplemented by additional tests such as:

• Pterostilbene assay,
• Total polyphenol content,
• Total flavonoid content,
• Anthocyanin assay,
  to establish a complete antioxidant profile of the sample.

Industrial and regulatory applications

Resveratrol assay is used in many sectors:

• Nutraceuticals : control of resveratrol content in food supplements and plant extracts for health or anti-aging purposes.
• Food processing and oenology : monitoring of polyphenol levels in wine, grapes or juices, an indicator of quality and antioxidant activity.
• Natural cosmetics : formulation of anti-aging and antioxidant active ingredients in skincare.
• Scientific research : studies of bioavailability, antioxidant efficacy and synergistic interactions with other phenolic compounds (quercetin, catechins, pterostilbene).

From a regulatory standpoint, extracts standardized in resveratrol must meet purity and traceability requirements, particularly within the framework of European regulations on food ingredients and supplements .

Carrying out analyses at YesWeLab

YesWeLab collaborates with a network of expert laboratories in chromatography of plant polyphenols and metabolites HPLC-DAD , UHPLC and LC-MS/MS instruments ISO 17025 requirements .

The tests are performed using validated methods, ensuring accuracy , reproducibility , and complete traceability of the results. Analysis reports include:

• the total resveratrol content (mg/g or mg/L) ,
• the trans/cis distinction ,
• and compliance recommendations for industrial applications.

nutraceutical , agri-food and natural cosmetics players in the analytical control of their polyphenolic extracts and in the valorization of their active ingredients.

Thanks to its digital platform , YesWeLab centralizes your analytical needs, from quote request to results receipt, guaranteeing responsive and personalized scientific support.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors: Nutraceuticals, food processing, plants, natural cosmetics
• Measured parameters: Resveratrol (trans and cis)
• Normative references: ISO 17025, validated internal methods

For any polyphenol analysis or antioxidant profile development needs, contact our scientific team for a personalized quote.