Rebaudioside C dosage

Rebaudioside C is a steviol glycoside found in the leaves of Stevia rebaudiana , alongside rebaudioside A and stevioside. Less sweet but more balanced in terms of sensory quality, it contributes to the characteristic taste profile of stevia extracts.

The analysis of rebaudioside C, carried out by HPLC-DAD or LC-MS/MS , makes it possible to quantify this natural compound in crude extracts, refined sweeteners or sweetening formulations. This measurement is essential for standardizing stevia-based products , ensuring their taste consistency and verifying their regulatory compliance .

Rebaudioside C: a steviol glycoside with a specific sweetness profile

Rebaudioside C belongs to the family of glycosylated diterpenes derived from steviol, responsible for the sweet taste of the Stevia rebaudiana . Its chemical structure, slightly different from that of rebaudioside A , influences its sweetness intensity and aromatic profile.

• Sweetening power : approximately 30 to 50% of that of rebaudioside A.
• Sensory benefit : less bitter and rounder taste, often used to sweeten natural sweetener blends.
• Natural presence : mainly in varieties of Stevia rebaudiana Bertoni rich in secondary glycosides.

This compound is particularly studied for its technological and organoleptic interest , notably in the formulation of sweeteners with a more balanced taste and in the search for natural substitutes for sugar without glycemic effect.

Objectives and interest of the dosage of rebaudioside C

The dosage of rebaudioside C aims to:

• determine the glycosidic composition of stevia extracts,
• standardize the production of natural sweeteners according to their sensory profile,
• verify the purity and conformity of products intended for human consumption,
• monitor the stability of steviol glycosides during purification processes,
• validate the claims “natural sweetener” and “no added sugar”.

The most frequently analyzed matrices include:

• raw or purified stevia extracts ,
• powders and syrups ,
• drinks and diet products ,
• nutraceutical formulations and food supplements.

Analytical methods used

Rebaudioside C is analyzed by HPLC-DAD or LC-MS/MS , two reference methods for the characterization of steviol glycosides.

• HPLC-DAD : preferred technique for routine assays, allowing the separation of different glycosides on a C18 column and their detection at 210 nm. It offers excellent precision for standardized extracts.
• LC-MS/MS : more sensitive and specific method, used to confirm the structure of rebaudioside C and distinguish glycosidic isomers in complex matrices.

Results can be expressed as percentage purity or mg/g of product.

This analysis is often coupled with other complementary tests, including:

• Rebaudioside A dosage,
• Stevioside dosage,
• Determination of total steviol glycosides,
• Glucose and fructose dosage , to assess the overall composition of sugars and sweetener substitutes.

Industrial and regulatory applications

The analysis of rebaudioside C is essential in several contexts:

• Food industry : quality control of stevia extracts used in drinks, desserts, confectionery and light products.
• Nutraceuticals and food supplements : standardization of stevia-based formulations, particularly those claiming a hypoglycemic or metabolic effect.
• Regulation and compliance : verification of compliance with the specifications of Regulation (EU) No 231/2012 on steviol glycosides (E960) and the Codex Alimentarius .
• Research and innovation : study of glycosidic profiles to develop new natural sweeteners with better stability and optimized sweet taste.

These analytical controls ensure safety of use , transparency of labeling and control of the organoleptic quality of products containing stevia extracts.

Carrying out analyses at YesWeLab

YesWeLab relies on a network of partner laboratories specialized in the chromatography of glycosidic compounds high-performance HPLC-DAD and LC-MS/MS instruments

The tests are carried out according to protocols compliant with ISO 17025 , guaranteeing the reliability, traceability and reproducibility of the results.

The YesWeLab scientific team supports manufacturers in nutrition , natural ingredients and food research in:

• the selection of suitable analytical methods,
• validation of batches of extracts,
• and the technical interpretation of the results.

Since 2020, YesWeLab has contributed to the promotion of natural sweeteners by offering recognized analytical expertise and an intuitive digital platform for the complete management of analyses.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors: Nutraceuticals, food, natural ingredients, plants
• Measured parameters: Rebaudioside C, total steviol glycosides
• Normative references: ISO 17025, Regulation (EU) No. 231/2012, Codex Alimentarius

For any specific request or comparative study between steviol glycosides, contact our scientific team for a personalized quote and tailor-made technical support.