Rebaudioside A dosage

Rebaudioside A is a steviol glycoside extracted from the leaves of Stevia rebaudiana , a plant native to South America known for its strong natural sweetening power . This compound, up to 300 times sweeter than sucrose, is used as a low-calorie sweetener in foods, beverages, and nutritional supplements.

Rebaudioside A is measured using HPLC-DAD (high-performance liquid chromatography with UV detection) or LC-MS/MS for precise and selective quantification. This analysis ensures the purity, regulatory compliance, and standardization of stevia-based products for the food and nutraceutical industries.

Rebaudioside A: a benchmark natural sweetener

Rebaudioside A is the main sweetening compound in the Stevia rebaudiana . It belongs to the family of glycosylated diterpenes derived from steviol , which give stevia its characteristic sweet taste without significant calorie intake.

Among the steviol glycosides, rebaudioside A is one of the purest and most palatable, thanks to its low residual bitterness . It is commonly used as a sugar substitute in:

• soft drinks,
• dairy products and desserts,
• confectionery and chewing gum,
• food supplements,
• and nutraceutical formulations for metabolic purposes.

Objectives and interest of the dosage of rebaudioside A

Analysis of rebaudioside A by chromatography allows:

• precisely quantify the steviol glycoside content in stevia extracts,
• verify the regulatory compliance of the sweeteners used (minimum purity, absence of impurities or adulteration),
• evaluate the relative composition between the different glycosides (rebaudiosides A, C, D, F, stevioside),
• standardize plant extracts intended for food or nutraceuticals,
• validate nutritional claims related to sugar or caloric reduction.

The affected matrices include:

• raw or refined stevia extracts,
• functional or light drinks,
• natural sweetening products (powders, syrups),
• plant-based nutraceutical formulations

Analytical methods used

The dosage of rebaudioside A is based on high-precision chromatographic techniques:

• HPLC-DAD : Reference method for the quantification of steviol glycosides in plant extracts and finished products. Analysis is carried out on a C18 column, with detection in the UV around 210 nm.
• LC-MS/MS : method of choice for complex matrices or low contents, offering specific identification and high sensitivity.

These analyses also make it possible to distinguish rebaudioside A from other structurally close molecules such as stevioside , rebaudioside C or dulcoside A , in order to ensure a complete characterization of the extract.

For a complete study of the sweetness profile, this service can be combined with:

• Stevioside dosage,
• Analysis of total steviol glycoside,
• Dosage of glucose and fructose,
• Sucrose determination.

Industrial and regulatory applications

The dosage of rebaudioside A is part of a strict regulatory framework defined by the health authorities (EFSA, FDA, ANSES).

Main applications include:

• Quality control of stevia extracts : verification of purity and steviol glycoside profile in accordance with the specifications of the Codex Alimentarius and Regulation (EU) No. 231/2012.
• Formulation of natural sweeteners : development of optimized sweetening blends combining rebaudioside A with other natural molecules (erythritol, inulin, mogroside glycosides, etc.).
• Dietary and sugar-free products : control of light formulations intended for diabetics or those in calorie control.
• Nutraceutical research and innovation : stability studies, interactions with other ingredients and bioavailability of steviol glycosides.

These analyses guarantee the safety of use , traceability and conformity of products containing stevia, by ensuring exact labeling of active concentrations.

Carrying out analyses at YesWeLab

YesWeLab collaborates with laboratories expert in analytical chemistry of natural glycosides HPLC-DAD and LC-MS/MS systems compliant with ISO 17025 .

Tests are performed according to validated protocols, adapted to the matrix and expected concentration. Reports include:

• the rebaudioside A content ,
• the overall profile of steviol glycosides ,
• and interpretive comments for regulatory compliance.

Since 2020, YesWeLab has been supporting manufacturers of nutrition, functional drinks and natural ingredients in the analytical control of their sweetening products.

Thanks to its digital platform , YesWeLab simplifies the ordering, monitoring and receipt of analyses, while guaranteeing the quality and responsiveness of the service.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors: Nutraceuticals, agrifood, plants, natural ingredients
• Measured parameters: Rebaudioside A, steviol glycosides
• Normative references: ISO 17025, Codex Alimentarius, Regulation (EU) No. 231/2012

To obtain a personalized quote or technical support on the compliance of your natural sweeteners, contact our scientific team today.