Protodioscin assay

Protodioscin , and physical performance, which explains its frequent presence in dietary supplements marketed for male wellness or athletic recovery.

Its concentration is an essential step in guaranteeing the quality, safety, and efficacy of products that claim it as an active ingredient. Analysis of protodioscin also helps prevent the risks of fraud or insufficient standardization of plant extracts.

Analytical methods

The analysis of protodioscin relies on reference chromatographic methods:

• HPLC (high-performance liquid chromatography) coupled with UV detection, allowing the separation and quantification of protodioscin in complex matrices.
• LC-MS/MS (liquid chromatography coupled with mass spectrometry) , used for more specific detection and sensitive quantification, even at very low concentrations.

These methods guarantee reliable and reproducible results, in line with the expectations of nutraceutical and phytotherapy manufacturers.

Affected matrices

The measurement of protodioscin is relevant in several types of samples:

• Standardized plant extracts of Tribulus terrestris.
• Raw materials used in nutraceutical formulations.
• Capsules, powders and tablets of food supplements.
• Finished products marketed in the field of natural health.

Industrial and scientific applications

Analytical control of protodioscin addresses various needs:

• Standardization of extracts to ensure a constant content of active ingredient.
• Quality control to verify the conformity of raw materials and finished products.
• Safety and efficacy in the context of health and nutritional claims.
• Research and development for the development of new plant-based formulations with a functional purpose.

Related analyses in the YesWeLab catalog

Protodioscin testing can be complemented by other analytical services available on the YesWeLab website:

• Saponin assay for a global evaluation of compounds present in extracts of Tribulus and other plants.
• Assay of diosgenin for other steroidal saponins of nutraceutical interest.
• Flavonoid assay to complete the analysis of the plant's bioactive profile.
• Hesperidin assay and rutin assay , often combined in antioxidant formulations.

Regulations and issues

In the nutraceutical sector, standardizing Tribulus terrestris extracts and controlling protodioscin content are essential to meet regulatory and marketing expectations. Some countries impose specific limits or recommendations regarding the composition of saponin-based food supplements.

Furthermore, analytical quality guarantees consumer confidence and allows products to be valued in highly competitive markets.

YesWeLab expertise

YesWeLab relies on a network of partner laboratories accredited to ISO 17025 and/or COFRAC standards, selected for their technical expertise and their ability to meet the specific requirements of each client. Through an innovative digital platform, we facilitate the management of analytical requests, from sample submission to results receipt.

Our scientific team supports manufacturers, distributors and formulators in setting up appropriate analytical plans, guaranteeing accuracy, reliability and regulatory compliance.