Progoitrin (glucosinolate) dosage

Progoitrin is a natural glucosinolate found in plants of the Brassicaceae , including rapeseed ( Brassica napus ) , mustard, and turnip seeds. These sulfur compounds play a key role in the natural defense of plants against insects and pathogens. However, excessive progoitrin levels can pose a health risk, particularly by altering thyroid function through inhibition of hormone synthesis.
nutraceutical and agri-food sectors , progoitrin measurement is an essential step in ensuring the safety , quality , and standardization of plant raw materials and functional extracts. This analysis makes it possible to control the conformity of finished products and to secure formulations intended for human or animal consumption.

Analytical methods used

The analysis of progoitrin is based on high-precision chromatographic techniques, adapted to the detection of sulfur compounds present at low concentrations:

• HPLC (High Performance Liquid Chromatography) : this method, coupled with UV detection, allows the separation and quantification of glucosinolates.
• LC-MS/MS (Liquid Chromatography coupled with Tandem Mass Spectrometry) : this approach offers high specificity and sensitivity , allowing the detection of progoitrin at trace levels, even in complex matrices.

These techniques, widely used in YesWeLab's partner laboratories, guarantee reproducible , traceable that comply with the most demanding analytical standards.
To learn more about chromatographic principles, also visit the page dedicated to high-performance liquid chromatography (HPLC) .

Affected matrices

Progoitrin assay can be applied to many matrices from glucosinolate-rich plants:

• Rapeseed, mustard or turnip seeds.
• Rapeseed cake from the crushing of oilseeds.
• Standardized or concentrated plant extracts.
• Finished products for nutraceutical or functional food .

These analyses allow the progoitrin concentration to be monitored throughout the processing chain, from raw material to finished product, guaranteeing consistent quality.

Industrial and scientific applications

The analysis of progoitrin is of strategic importance in several industrial contexts:

• Food and nutritional safety : control of goitrogenic compound levels to avoid any toxic risk.
• Animal nutrition : monitoring of glucosinolates in rapeseed meal, to ensure safe formulations for livestock.
• Standardization of plant extracts : essential for the production of food supplements based on Brassicaceae.
• Plant research and innovation : studies on the biosynthesis of glucosinolates, their biological role and the selection of low-content varieties.

This analytical approach is often integrated into a more global program of evaluation of phytochemical compounds, combined with other analyses such as the dosage of total glucosinolates , the dosage of goitrin or the dosage of total polyphenols to characterize the associated antioxidant properties.

Regulation and compliance issues

Progoitrin testing is part of a strict regulatory framework. Glucosinolates are subject to recommended thresholds for plant foods and products intended for animal feed.
Excessive concentrations can affect the digestibility, nutritional value, or safety of formulations. The analyses conducted therefore make it possible to:

• Demonstrate regulatory compliance of raw materials.
• Guarantee batch traceability
• Meet the requirements of specifications in nutrition, nutraceuticals and phytotherapy.

In a market where naturalness, quality and safety are major differentiating criteria, controlling progoitrin content is a strategic asset for manufacturers.

YesWeLab expertise

YesWeLab relies on a network of carefully selected partner laboratories , accredited to ISO 17025 and/or COFRAC, guaranteeing reliable and compliant analytical services. Our experts assist you in:

• Choosing the method suited to your matrix (HPLC, LC-MS/MS).
• Interpretation of results and validation of regulatory thresholds.
• The integration of these analyses into your quality control protocols or your R&D studies .

Thanks to its digital platform , YesWeLab facilitates the complete management of your analyses: request for quotes, sample tracking and consultation of results online.

For any specific request or study project, contact our scientific team to benefit from tailor-made support in the dosage of progoitrin and other glucosinolates.