Polymorphism assay

polymorphisms , particularly SNPs (Single Nucleotide Polymorphisms), are sequence variations present in DNA between individuals of the same species. Their detection and measurement make it possible to identify genetic markers useful for traceability, selection and improvement of biological performance .

In agriculture and animal breeding, these analyses offer the possibility of identifying genes of interest associated with disease resistance, productivity, or the quality of raw materials. In the cosmetics field, the detection of polymorphisms can be used to develop personalized approaches based on skin genetics.

Analytical methods

The dosage of polymorphisms is based on different complementary approaches:

• Real-time PCR (qPCR) : Allows rapid detection of specific polymorphisms in targeted samples.
• Sanger sequencing : classic method for confirming point variations.
• NGS (Next Generation Sequencing) : offers broader coverage to identify and quantify many polymorphisms in a single analysis.
• Genotyping by specific probes : solution adapted to large-scale studies or panels of individuals.

These techniques guarantee precision and reproducibility, while adapting to the needs of research or industrial projects.

Affected matrices

The dosage of polymorphisms can be carried out on different types of biological matrices:

• DNA extracted from plants for varietal improvement and seed certification
• Yeast and microorganism DNA for biotechnology and fermentations
• Animal DNA for genetic selection, traceability and veterinary research
• Human DNA in the context of personalized cosmetics or non-medical research

Industrial and scientific applications

• Agriculture and plant breeding : identification of varieties resistant to biotic or abiotic stresses.
• Animal health and breeding : monitoring of polymorphisms associated with robustness and productivity.
• Personalized cosmetics : DNA analyses to offer products adapted to the skin's genetic profile.
• Biotechnologies and fermentation : monitoring of yeast or bacterial strains to optimize processes.
• Genetic traceability : certification of the origin and authenticity of organic products.

Additional analyses

For a comprehensive approach, the polymorphism assay can be combined with other analyses available in the YesWeLab catalog:

• DNA sequencing (NGS) for in-depth genetic identification.
• Dosage of methanogenic flora useful in environmental and fermentation processes.

Regulations and issues

The detection of polymorphisms is governed by quality standards, particularly in the agricultural and food sectors, to ensure compliance with traceability and labeling regulations. In the cosmetics sector, it is part of an innovative approach, where genetics becomes a lever for differentiation for personalized care.

These analyses also contribute to fundamental research in genomics and population biology, providing valuable data for understanding genetic diversity.

YesWeLab expertise

YesWeLab provides industry and researchers with its network of ISO 17025-accredited partner laboratories certified for molecular biology methods. Each project benefits from a tailored approach, adapted to the nature of the matrix, the detection method, and regulatory requirements.

Since 2020, YesWeLab has been supporting players in agriculture, animal health, biotechnology and cosmetics with their analytical needs, by offering them a centralized digital platform to easily manage their samples and results.